Protein Info for ABID97_RS20025 in Variovorax sp. OAS795

Annotation: D-glycerate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 327 PF00389: 2-Hacid_dh" amino acids 7 to 320 (314 residues), 95.7 bits, see alignment E=3.7e-31 PF02826: 2-Hacid_dh_C" amino acids 109 to 291 (183 residues), 191.7 bits, see alignment E=1.5e-60 PF03446: NAD_binding_2" amino acids 148 to 252 (105 residues), 21.7 bits, see alignment E=3.7e-08 PF03807: F420_oxidored" amino acids 148 to 218 (71 residues), 23.9 bits, see alignment E=1.1e-08

Best Hits

Swiss-Prot: 51% identical to GHRB_CITK8: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 96% identity to vap:Vapar_3413)

MetaCyc: 55% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"2-ketogluconate 6-phosphate reductase (EC 1.1.1.43)" in subsystem 2-Ketogluconate Utilization (EC 1.1.1.43)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.43

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (327 amino acids)

>ABID97_RS20025 D-glycerate dehydrogenase (Variovorax sp. OAS795)
MTTKKNVLVFRPLPEDQLARLQAAHHVTVADPRKEPEAFAAALGTANGLIGASHTVDAAL
LDAAPALEVISSISVGVDNYALAELHKRGIVLCHTPDVLTETVADTVFAILMATQRRVVE
LSNLVREGRWTKNIGDELFGTDVHGKTLGILGFGRIGQAVARRAALGFGMPVLYHSRRPV
DLAAQAPELQGRATHTPLDALLARSDIVLAMLPLTDATRGMIDAAFFARMKAGAAFINGG
RGATVDEAALLHALDHGTLRAAGLDVFAKEPLPADSPLRTHPRVTPLPHIGSATHETRHA
MAELATTNLLQVLAGETPTAAYDTAAA