Protein Info for ABID97_RS19370 in Variovorax sp. OAS795

Annotation: isocitrate lyase/PEP mutase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 PF13714: PEP_mutase" amino acids 12 to 253 (242 residues), 157.3 bits, see alignment E=5.3e-50 PF00463: ICL" amino acids 82 to 174 (93 residues), 42.6 bits, see alignment E=3.1e-15

Best Hits

Swiss-Prot: 42% identical to CPPM_STRHY: Carboxyvinyl-carboxyphosphonate phosphorylmutase (bcpA) from Streptomyces hygroscopicus

KEGG orthology group: K01003, carboxyvinyl-carboxyphosphonate phosphorylmutase [EC: 2.7.8.23] (inferred from 80% identity to ctt:CtCNB1_1260)

MetaCyc: 45% identical to carboxyphosphonoenolpyruvate phosphonomutase (Kitasatospora phosalacinea)
Carboxyvinyl-carboxyphosphonate phosphorylmutase. [EC: 2.7.8.23]; 2.7.8.23 [EC: 2.7.8.23]

Predicted SEED Role

"Methylisocitrate lyase (EC 4.1.3.30)" in subsystem Methylcitrate cycle or Propionate-CoA to Succinate Module (EC 4.1.3.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.30

Use Curated BLAST to search for 2.7.8.23 or 4.1.3.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>ABID97_RS19370 isocitrate lyase/PEP mutase family protein (Variovorax sp. OAS795)
MPTNDESPKKTLRRLAEARRGVLVPGAFNALSARVIEDLGFEAIYVTGAGVTNMHFGLPD
QAFMGLHDIAEHTARIRDAVNLPLLVDADTGFGNALNVRHAVRVLERAGADCIQLEDQVS
PKRCGHFAGKAVIGTEEMLGKIKAAVDARTDAGMLVMARTDAAAVHGFEAAIERAQQFSE
AGADILFVEAVTQADEVRAMPQRLDKPQLMNMVIGGQTPTFGAEELGALGYGLVLYANAA
LQGAVAGMQRALTVLRDSRRLDEDPSLVVSFAERQRLVGKPWLDELAQRYAG