Protein Info for ABID97_RS17525 in Variovorax sp. OAS795

Annotation: acetate--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02188: acetate--CoA ligase
  • amino acids 32 to 663 (632 residues), 945.3 bits, E=7.2e-289
• PF16177: ACAS_N
  • amino acids 36 to 91 (56 residues), 72.2 bits, 3.8e-24
• PF00501: AMP-binding
  • amino acids 102 to 484 (383 residues), 299.2 bits, E=6e-93
• PF13193: AMP-binding_C
  • amino acids 548 to 630 (83 residues), 90.1 bits, E=2.1e-29

Best Hits

Swiss-Prot: 76% identical to ACSA_CUPMC: Acetyl-coenzyme A synthetase (acsA) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 96% identity to vpe:Varpa_3406)

MetaCyc: 76% identical to acetyl-CoA synthetase (Cupriavidus necator)
Propionate--CoA ligase. [EC: 6.2.1.17]; Acetate--CoA ligase. [EC: 6.2.1.17, 6.2.1.1]

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of coenzyme A biosynthesis II (plants) (9/10 steps found)
• 2-methylcitrate cycle I (5/5 steps found)
• acrylate degradation II (3/3 steps found)
• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• superpathway of acetate utilization and formation (3/3 steps found)
• β-alanine biosynthesis II (5/6 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation III (2/3 steps found)
• 2-methylcitrate cycle II (4/6 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• chitin deacetylation (2/4 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (4/9 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (3/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1, 6.2.1.17

Use Curated BLAST to search for 6.2.1.1 or 6.2.1.17

Protein Sequence (665 amino acids)

>ABID97_RS17525 acetate--CoA ligase (Variovorax sp. OAS795)
MSSASKNIESVLVENRVFPPDARASAGARISGMAAYDALCKEAEQDFEGFWSRLAKDNLQ
WTKPFTRTLDESKAPFYEWFGDGELNASANCLDRHIGTPVENKTAIVFEADDGAVTRVTY
KELLARVSQFANALKAAGIQKGDRVIVYMPMTVEGVIAMQACARIGATHSVVFGGFSAKA
LQERIIDAGAVAVITANYQLRGGKELPLKAIVDDGIAMGGCESIKTVFVYERTPTAWNRV
EGRDKSFAEALQGQSTDCAPVPVNAEHPLFILYTSGSTGKPKGVQHATGGYLLWAKLTMD
WTFDIRPEDVFWCTADIGWITGHTYVAYGPLAAGATQVVFEGIPTFPHAGRFWQMIEQHK
VSVFYTAPTAIRSLIKAAEGDEKVHPKNWDLSSLRILGSVGEPINPEAWMWYHRNIGREQ
CPIVDTFWQTETGGHVITPLPGATPLVPGSCTLPLPGIMAAIVDETGKDLPNGAGGMLVI
KRPWPSMIRTIWNDPERFKKSYFPEEMGGTIYLAGDGAVRSADRGYFRITGRIDDVLNVS
GHRLGTMEIESALVSKTDLVAEAAVVGRPDDVTGEAVCAFVVLKRSRPTGEEARQIANEL
RNWVAKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSIAKGEAITQDTSTLENPAILDQ
LSQTN