Protein Info for ABID97_RS17080 in Variovorax sp. OAS795

Annotation: carbamoyl-phosphate synthase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01369: carbamoyl-phosphate synthase, large subunit
  • amino acids 2 to 1059 (1058 residues), 1530.7 bits, E=0
• PF02786: CPSase_L_D2
  • amino acids 135 to 341 (207 residues), 240.2 bits, E=6.8e-75
  • amino acids 675 to 881 (207 residues), 127.8 bits, E=1.7e-40
• PF02787: CPSase_L_D3
  • amino acids 428 to 506 (79 residues), 95.2 bits, 7.5e-31
• PF02222: ATP-grasp
  • amino acids 685 to 852 (168 residues), 41.3 bits, E=5.2e-14
• PF15632: ATPgrasp_Ter
  • amino acids 752 to 881 (130 residues), 31.5 bits, E=5.4e-11
• PF02142: MGS
  • amino acids 963 to 1049 (87 residues), 79.1 bits, E=8.8e-26

Best Hits

Swiss-Prot: 66% identical to CARB_BUCAP: Carbamoyl-phosphate synthase large chain (carB) from Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)

KEGG orthology group: K01955, carbamoyl-phosphate synthase large subunit [EC: 6.3.5.5] (inferred from 88% identity to aaa:Acav_2592)

MetaCyc: 69% identical to carbamoyl-phosphate synthetase large subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Protein Sequence (1082 amino acids)

>ABID97_RS17080 carbamoyl-phosphate synthase large subunit (Variovorax sp. OAS795)
MPKRSDLQSILIIGAGPIIIGQACEFDYSGVQACKALREEGYKVILINSNPATIMTDPAT
ADVTYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDLWRNGVLDKYTGAATNKPV
ELIGATPEAIDKAEDRLKFKDAMTKIGLGSARSGIAHSMDEAWAVQKSVGFPTVIRPSFT
LGGTGGGIAYNPEEFETICKRGIEASPTNELLIEESLLGWKEYEMEVVRDKADNCIIVCS
IENLDPMGVHTGDSITVAPAQTLSDKEYQILRNASLAVLREIGVDTGGSNVQFSINPKDG
RMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELRNEITGGATPASFEPSID
YVVTKIPRFAFEKFPQADSRLTTQMKSVGEVMAMGRTFQESFQKALRGLEVGVDGLNEKT
QDREVLEKELGEPGPERIWYVGDAFAMGLSVDEVFALTKIDPWFLVQIEEIVKIELELET
KSLADIDKDTLLALKKKGFSDRRLAKQLKTTDTAVREKRRALGVRPVYKRVDTCAAEFAT
NTAYMYSTYEDECEANPTDKKKIMVLGGGPNRIGQGIEFDYCCVHAALAMREDGYETIMV
NCNPETVSTDYDTSDRLYFEPLTLEDVLEIVDKEKPLGVIVQYGGQTPLKLALDLEANGV
PIIGTSPDMIDAAEDRERFQKLLHELKLLQPPNATARTEPEALEKAAALGYPLVVRPSYV
LGGRAMEIVHEQRDLERYMREAVKVSNDSPVLLDRFLNDAVECDVDCLRDAEGQTLIGGV
MEHIEQAGVHSGDSACSLPPYSLSAETIAELKRQSAAMAAALNVVGLMNVQFAIQQKDGK
DVIYVLEVNPRASRTVPYVSKATGIQLAKVAARCMAGQSLKSQGVTKEVTPPYFSVKEAV
FPFVKFPGVDTILGPEMKSTGEVMGVGKTFGEAFVKSQLGAGTHLPKSGKVFISVKNNDK
ARAVEVARGLVKLGFEIIATKGTAAAIGAAGIECATVNKVTEGRPHIVDMIKNNEIALVI
NTVEERRNAIADSRQIRTSALLARVTTFTTIFGAEAAVEGMGFMDELGVISVQEMHAQLA
AA