Protein Info for ABID97_RS17005 in Variovorax sp. OAS795

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 506 PF02737: 3HCDH_N" amino acids 8 to 185 (178 residues), 195.6 bits, see alignment E=7.2e-62 PF00725: 3HCDH" amino acids 188 to 285 (98 residues), 89.3 bits, see alignment E=2e-29 amino acids 415 to 495 (81 residues), 58.9 bits, see alignment E=6e-20

Best Hits

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 94% identity to vap:Vapar_2632)

MetaCyc: 55% identical to 3-hydroxyacyl-CoA dehydrogenase (Cupriavidus necator H16)
3-hydroxyacyl-CoA dehydrogenase. [EC: 1.1.1.35]

Predicted SEED Role

"3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 1.1.1.35, EC 4.2.1.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (506 amino acids)

>ABID97_RS17005 3-hydroxyacyl-CoA dehydrogenase (Variovorax sp. OAS795)
MTVNYKRIGVVGAGAMGRGIAQIAAQAGSDVLLLDSFAGAAERGREALAAQWNKLHEKGK
IDAAARDAQIARVKAVGSVADLAPCDLVIEAVVEDLEVKRSLFRELEAVVAPTATLVTNT
SSLSVTAIAAVLERPERVAGFHFFNPVPLMKVVEVVAGFKTSADVCKQLAGYAVQMGHSA
VQAQDTPGFIVNHAGRGYGTEALRIVGEGVADFATVDRILKEQAGFRLGPFELLDLTALD
VSHPVMESIYRQYYEEPRFRPSVITAQRLAAGVLGRKTNEGFYKYHDGVMQQVPEAAPPA
VAALPAVWVSPRAARRADLLRLVNALGAQIDSGAAAASTSLILVAPLGFDVTTVAAVERL
DATRTVGIDMLIDDAATRRRVLATNPATRRDIRDAAHALFARDGKAVSVIRDSGGFVTQR
VIGTIVNIAADMCQQRVCSPADLETAVQLGLGYPRGPLAMGNLYGPTNMLEVLFNLQTVY
GDPRYRPSPWLRRRGALGLSLLHEEE