Protein Info for ABID97_RS16910 in Variovorax sp. OAS795

Annotation: ATP-dependent Clp protease ATP-binding subunit ClpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 773 TIGR02639: ATP-dependent Clp protease ATP-binding subunit ClpA" amino acids 3 to 742 (740 residues), 1098.2 bits, see alignment E=0 PF02861: Clp_N" amino acids 13 to 62 (50 residues), 54.4 bits, see alignment 6.7e-18 PF00004: AAA" amino acids 219 to 351 (133 residues), 48.4 bits, see alignment E=7.8e-16 amino acids 500 to 623 (124 residues), 45.8 bits, see alignment E=5e-15 PF17871: AAA_lid_9" amino acids 359 to 456 (98 residues), 90.8 bits, see alignment E=3e-29 PF07724: AAA_2" amino acids 494 to 654 (161 residues), 199.3 bits, see alignment E=2.8e-62 PF00158: Sigma54_activat" amino acids 498 to 620 (123 residues), 23 bits, see alignment E=3.5e-08 PF07728: AAA_5" amino acids 499 to 617 (119 residues), 48.8 bits, see alignment E=4.6e-16 PF10431: ClpB_D2-small" amino acids 661 to 740 (80 residues), 79.4 bits, see alignment E=1e-25

Best Hits

Swiss-Prot: 61% identical to CLPA_ECO57: ATP-dependent Clp protease ATP-binding subunit ClpA (clpA) from Escherichia coli O157:H7

KEGG orthology group: K03694, ATP-dependent Clp protease ATP-binding subunit ClpA (inferred from 91% identity to aaa:Acav_2010)

MetaCyc: 61% identical to ATP-dependent Clp protease ATP-binding subunit ClpA (Escherichia coli K-12 substr. MG1655)
Endopeptidase Clp. [EC: 3.4.21.92]

Predicted SEED Role

"ATP-dependent Clp protease ATP-binding subunit ClpA" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.92

Use Curated BLAST to search for 3.4.21.92

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (773 amino acids)

>ABID97_RS16910 ATP-dependent Clp protease ATP-binding subunit ClpA (Variovorax sp. OAS795)
MIAQELEVSLHMAFVEARQQRHEFITVEHLLLALLDNPSAAEVLRACSANVDDLRASLTN
FIKDNTPQVAGTDDVDTQPTLGFQRVIQRAIMHVQSTGNGKKEVTGANVLVAIFGEKDSH
AVYYLHQQGVTRLDVVNFIAHGIKKSDPPEAVKGSSESSSGEGEEGGGEKNEKASPLEQF
TQNLNQLAKDGKIDPLIGREYEVERVIQILCRRRKNNPLLVGEAGVGKTAIAEGLAWRIT
QADVPEILAEAQVYSLDMGALLAGTKYRGDFEQRLKGVLKSLKDKPNAILFIDEIHTLIG
AGAASGGTLDASNLLKPALSSGQLKCIGATTFSEYRGIFEKDAALSRRFQKVDVVEPTVQ
ETVDILKGLKSRFEEHHGVKYAVAALQAAAELSAKYINDRHLPDKAIDVIDEAGAAQRIL
PANKRKKTISKTEVEDIVAKIARIPPANVSNDDRGKLQTIERDLKSVVFGQDKALEVLAS
AVKMARSGLGREDKPIGSFLFSGPTGVGKTEAAKQLAYIMGIELIRFDMSEYMERHAVSR
LIGAPPGYVGFDQGGLLTEAITKKPHAVLLLDEIEKAHPDIFNVLLQVMDHGTLTDNNGR
KADFRNVIIVMTTNAGAETMNKATIGFTNPRQAGDEMADIKRLFTPEFRNRLDATVSFKA
LDEQIILRVVDKFLLQLETQLAEKKVDVTFSDGLRKHLAKKGFDPLMGARPMQRLIQDTI
RRALADELLFGRLIDGGRLSVDIDDKGEVQLDIQPLPKKEGKSKPEAEEAAAG