Protein Info for ABID97_RS16695 in Variovorax sp. OAS795

Annotation: Tol-Pal system beta propeller repeat protein TolB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 408 TIGR02800: Tol-Pal system beta propeller repeat protein TolB" amino acids 4 to 403 (400 residues), 484.8 bits, see alignment E=1.2e-149 PF04052: TolB_N" amino acids 9 to 108 (100 residues), 102.8 bits, see alignment E=2.2e-33 PF07676: PD40" amino acids 172 to 204 (33 residues), 25.4 bits, see alignment (E = 2.1e-09) amino acids 213 to 247 (35 residues), 28.7 bits, see alignment 1.9e-10 amino acids 256 to 292 (37 residues), 45.8 bits, see alignment 8.2e-16 amino acids 301 to 335 (35 residues), 26.4 bits, see alignment 1e-09 amino acids 347 to 369 (23 residues), 12.6 bits, see alignment (E = 2.2e-05)

Best Hits

Swiss-Prot: 81% identical to TOLB_RHOFT: Tol-Pal system protein TolB (tolB) from Rhodoferax ferrireducens (strain ATCC BAA-621 / DSM 15236 / T118)

KEGG orthology group: K03641, TolB protein (inferred from 98% identity to vap:Vapar_2692)

Predicted SEED Role

"tolB protein precursor, periplasmic protein involved in the tonb-independent uptake of group A colicins" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (408 amino acids)

>ABID97_RS16695 Tol-Pal system beta propeller repeat protein TolB (Variovorax sp. OAS795)
MLPALAQFRVEVSGVGLTQRPIALVPFKGQDASPQKISDIVQADLERSGQFRGVDASGQA
LDEASRPDLALWRQRTADSLVVGSVTRLADGRFDVRFRLWDVVRGQDLGGQSYTVPQGDL
RLASHRIADYVYEKLTGDKGIFSTRIAYVTKGGSRYSLWVADADGENAQAALASPEPIIS
PVWSSNGQQLAYVSFESRKPVVYVHNVASGQRRLLANFRGSNSAPAWAPDGGSLAVTLSR
DGGSQLYTIPASGGEPRRLTQSASIDTEPVYSADGSTIYFVSDRGGAPQIYKMGASGGAP
TRVTFSGTYNISPSVSGDGRWLAYISRVGGAFKLHVMELASGNVSAITDTTADESPSFAP
NSKLIVYATQLQGREALMTTTLDGKIKARLAGQAGDIREPDWGPFQKQ