Protein Info for ABID97_RS16650 in Variovorax sp. OAS795

Annotation: 50S ribosomal protein L3 N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 1 to 283 (283 residues), 326.9 bits, see alignment E=1.1e-101 TIGR00536: methyltransferase, HemK family" amino acids 14 to 283 (270 residues), 175.1 bits, see alignment E=1.7e-55 PF06325: PrmA" amino acids 121 to 195 (75 residues), 23.3 bits, see alignment E=8.1e-09 PF05175: MTS" amino acids 121 to 202 (82 residues), 59.3 bits, see alignment E=7.5e-20 PF13847: Methyltransf_31" amino acids 123 to 196 (74 residues), 29.1 bits, see alignment E=1.5e-10 PF13649: Methyltransf_25" amino acids 125 to 201 (77 residues), 30.9 bits, see alignment E=7.1e-11

Best Hits

Swiss-Prot: 46% identical to PRMB_NEIMB: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 94% identity to vap:Vapar_2716)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>ABID97_RS16650 50S ribosomal protein L3 N(5)-glutamine methyltransferase (Variovorax sp. OAS795)
MSTVIELIEAGAKRLEDAGVAFGHGTANAFDEAAWLVLWRLKLPLDGIDAVADTPVSPAD
AGKVAALLDERIATRKPAAYLTKEAWLQGVPFYVDERAIVPRSFIAELMADGSIDYWLGE
HTQRVLDLCTGNGSLAVLAAMTYPEITVDAADLSVEALEVAAINVTRHQLDSRIRLVESD
GLANLPGPYDLVLCNPPYVNSASMAALPAEYRAEPELALAGGTDGMDFVRRLFADAPSRM
SEEAVLVLEIGNERDYFEAAFPKLEVVWLETSAGEDQVLLVTRAALLAGAGAR