Protein Info for ABID97_RS16530 in Variovorax sp. OAS795

Annotation: DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF01068: DNA_ligase_A_M" amino acids 48 to 204 (157 residues), 31.5 bits, see alignment E=1.5e-11 PF14743: DNA_ligase_OB_2" amino acids 218 to 283 (66 residues), 93.9 bits, see alignment E=4.7e-31

Best Hits

Swiss-Prot: 35% identical to DNLI_HAEIN: DNA ligase (ligA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 86% identity to vap:Vapar_2739)

Predicted SEED Role

"DNA ligase (ATP) (EC 6.5.1.1)" (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>ABID97_RS16530 DNA ligase (Variovorax sp. OAS795)
MRGGVRLTRRLLLLAGVGVLAARAATAREAPPALMLAEAYRPGMALADYWVSEKYDGVRG
YWDGTRLWTRGGEPVMAPAWFTARLPKQPLDGELWAGRGQFALAVATVRSQTPNDTAWHS
MRFMVFDLPGHGGEFTARLAALRKLLPITDAPWVVAVPQERATTPAELQALLDKTVKMGG
EGLMLHRGGSLYRAERTSDLLKVKLHEDAEARVVGHVPGKGRHGGRLGALLVETPEGKRF
KLGTGLTDAEREDPPALGSWVTYRFDGTHPGGLPRFARFVRVRLDLPSS