Protein Info for ABID97_RS14565 in Variovorax sp. OAS795

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 559 PF00027: cNMP_binding" amino acids 35 to 119 (85 residues), 48.4 bits, see alignment E=3.7e-16 PF07992: Pyr_redox_2" amino acids 230 to 541 (312 residues), 116.1 bits, see alignment E=1.1e-36 PF13450: NAD_binding_8" amino acids 234 to 267 (34 residues), 22.5 bits, see alignment (E = 5.6e-08) PF13738: Pyr_redox_3" amino acids 330 to 493 (164 residues), 36.2 bits, see alignment E=2.1e-12 PF00070: Pyr_redox" amino acids 379 to 448 (70 residues), 31.5 bits, see alignment E=1e-10

Best Hits

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 96% identity to vap:Vapar_2304)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (559 amino acids)

>ABID97_RS14565 FAD-dependent oxidoreductase (Variovorax sp. OAS795)
MATAASSNFDNRLHQTFPVLSDAEIARVAHFGTVQRFATGERLFTAGETSPGMFVLLKGV
VAVTQRDGLGHVVPIVRQGPGEFLAEVGQLSGRPSLVDGHAEEEVEALIVPPAQLRALLV
AEADLGERITRALILRRVALIESGASGPVLIGRPQSPDMARLENFLRRNGHPYHLVDAAE
DPDAAALLQQYGTCKLLAVCPDGSVLVNPAEDALARCLGMVDTAEHNELYDVAVVGAGPA
GLATAVYAASEGLRVIVLDCRAFGGQAGASARIENYLGFPTGISGQALAGRAFVQAQKFG
VEMLIPAKVTSLDCSRDNAMGSLRIALADGRAINARTVVIASGARYRRPDVPRLSEFEGR
GIWYWASAIEAKLCSQQEVALVGGGNSAGQAAVFLSRHAAKVNVLVRGPSLAASMSRYLI
DRIEATPNIELHPHTQLVKLNGGSEQGLTGATWRCQETGLEHDCAARNIFLFVGAEPETS
WLEGCSVSVDKHGFVRTGDAASAGFPARPATALESSVQGVFAVGDVRSGSVKRVGGAIGE
GAAVVALIHQHLSSNSAVA