Protein Info for ABID97_RS13985 in Variovorax sp. OAS795

Annotation: polyhydroxyalkanoate depolymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 471 TIGR01849: polyhydroxyalkanoate depolymerase, intracellular" amino acids 2 to 417 (416 residues), 570 bits, see alignment E=1.3e-175 PF06850: PHB_depo_C" amino acids 212 to 418 (207 residues), 301 bits, see alignment E=1.8e-94

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 96% identity to vap:Vapar_2138)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (471 amino acids)

>ABID97_RS13985 polyhydroxyalkanoate depolymerase (Variovorax sp. OAS795)
MLYQLYEAQRSLMEPFSDFAQAASKLFGQGAVWGQVPMAQRMAAGYDLLYRLGKDYEKPE
FNIKSVKVEGEDVVIQEAVELDKPFCELRRFKRFTDEPHILKTLKSQPVVLIVAPLSGHY
ATLLRDTVRTMLQDHKVYITDWKNARLVPLSEGEFHLDDYVNYVQEFIRRLQAEYGNCHV
VSVCQPTVPVLAAVSLMASRGEQLPLTMTMMGGPIDARKSPTAVNNLAMNKSFEWFENNV
IYRVPQGFPGEGRRVYPGFLQHTGFVAMNPDRHASSHYDYFKDLIKGDDASAEAHRKFYD
EYNAVLDMDADYYLDTIRAVFQEFDLVNGTWDVKNPKGQIERVRPQDIHSTALLTVEGEL
DDISGSGQTEAAHSLCTGIDDAQREHYEVKGAGHYGIFSGRRWRDLVYPKVVKFIAAHDQ
PQRRAGARESLPAEDRIKPARSTATAAARKNVAARKAVAVAPVKKTAARRK