Protein Info for ABID97_RS13610 in Variovorax sp. OAS795

Annotation: hydrogenase formation protein HypD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 382 TIGR00075: hydrogenase expression/formation protein HypD" amino acids 2 to 371 (370 residues), 472.7 bits, see alignment E=3.8e-146 PF01924: HypD" amino acids 12 to 369 (358 residues), 509.9 bits, see alignment E=1.9e-157

Best Hits

Swiss-Prot: 83% identical to HYPD_CUPNH: Hydrogenase maturation factor HypD (hypD) from Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337)

KEGG orthology group: K04654, hydrogenase expression/formation protein HypD (inferred from 90% identity to mpt:Mpe_A2812)

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypD" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (382 amino acids)

>ABID97_RS13610 hydrogenase formation protein HypD (Variovorax sp. OAS795)
MKYIDEFRDGAIAKKIQARLHAEAQPARQYSFMEFCGGHTHAISRYGIGELLPHNVRMIH
GPGCPVCVLPIGRIDLAIQLALERGAIVCTYGDTMRVPASGGLSLVRAKARGGDIRMVYS
GADALEIARREPGREVVFFAIGFETTTPPTALTIRTAQQEGLKNFSVLCCHVLTPSAITH
ILESPEVRQFGTVPIDGFVGPAHVSIVIGSAPYEHFAEEYRKPVVIAGFEPLDVMQAVLM
LVRQVNEGRAEVENEFTRAVNRDGNLHAQALVSQVFELRNSFEWRGLGEVPYSALKIRPA
FAEFDAERRFGLGYQPVPDNKACECGAILRGVKKPTDCKIFGTVCTPENPVGSCMVSSEG
ACAAHYSYGRFKDIAVKVEVAA