Protein Info for ABID97_RS13580 in Variovorax sp. OAS795

Annotation: nickel-dependent hydrogenase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 485 PF00374: NiFeSe_Hases" amino acids 38 to 117 (80 residues), 71.6 bits, see alignment E=3.4e-24 amino acids 243 to 485 (243 residues), 178.5 bits, see alignment E=1.3e-56

Best Hits

KEGG orthology group: None (inferred from 78% identity to mpt:Mpe_A2806)

Predicted SEED Role

"[Ni/Fe] hydrogenase, group 1, large subunit" in subsystem Hydrogenases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (485 amino acids)

>ABID97_RS13580 nickel-dependent hydrogenase large subunit (Variovorax sp. OAS795)
MTRLLVGPFNRVEGDLEVTLDIEDGRVSSARVNSPMYRGFEQILQGKRPHDALVYVPRIC
GICSVSQSVASARALADMGGLSIPPNGQHVTNVILATENMADHLTHFYLFFMPDFTRAVY
AGRPWHADAQRRFDPATGEQARQAIAARQRWFTMLGLLAGKWPHTQSIEPGGSTRAVDSA
ERTRLLARMREFRRFLEQQLFAAPLEQVLSLASADALWDWDARAPLRGDFRMFLAIARDA
GLMGLGKGPGRYLSYGAYPQPGAAFVFPRGVWRAESSSLGPLDESGIREDATHAWLADDG
GPRHPRDGLTVPAGDKPGAYTWNKAPRLAGEVVETGAIARQLAAGHPLVRDMVERHGGTV
FTRVLARLLELARVVPMMEQWMLALEPGEPYCASGALPDEGTGIGLSEAARGSLGHWISV
RRGHIENYQIVAPTTWNFSPRDAAGTPGALESALEGAPVLQGETTPVSVQHIVRSFDPCM
VCTVH