Protein Info for ABID97_RS12310 in Variovorax sp. OAS795

Annotation: thiamine pyrophosphate-requiring protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 593 PF02776: TPP_enzyme_N" amino acids 4 to 120 (117 residues), 112.5 bits, see alignment E=1.6e-36 PF00205: TPP_enzyme_M" amino acids 199 to 329 (131 residues), 97.6 bits, see alignment E=7.8e-32 PF02775: TPP_enzyme_C" amino acids 389 to 543 (155 residues), 97.3 bits, see alignment E=1.2e-31

Best Hits

KEGG orthology group: K00156, pyruvate dehydrogenase (quinone) [EC: 1.2.5.1] (inferred from 92% identity to vap:Vapar_1999)

Predicted SEED Role

"Pyruvate oxidase (EC 1.2.3.3)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.3.3 or 1.2.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (593 amino acids)

>ABID97_RS12310 thiamine pyrophosphate-requiring protein (Variovorax sp. OAS795)
MKQTVSDFVVQRLQDWGVRRVYGYPGDGINGLMGALDRAGGAIQFVQSRHEELAAFMACA
HAKFSGEVGVCLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQKRSALGGDYQQEVDLVS
LFKDVAHEFVQMATSAEQVRHLVDRAMRIARDRKAVTCVIFPNDVQELPAVPVPPREHGT
VHSGIGATARATVPDGVALQRAADIVNAGRKVAILAGAGALHATDALIEGADRLGAGVAK
ALLGKAAVPDDLPFVTGAIGVLGTRPSSRMMEECDTLLMVGSSFPYAEFLPPEGQARCVQ
IDTDPRALGLRYPAELGLVGDSRATLAALLPLLEPKAHGEWRQGIEADVAQWWRVLEARA
HVEASPLNPQRVFWELSSRLPDKSVLTCDSGTAANWFARDVKLRRGMKASLSGGLATMGP
ALPYAIAAKLVHPDRPVVAMLGDGAMQMNGLNALITLARMWRDWADPRLVVLVLNNRDLN
LVSWEQRVLGGDPKFDASQDLPDFPYARFAEMLGLAALRVDTPEAIGPAWDAAFASDRPM
LLEMVTDAAVPPLPPDVSMAQAKAYLSALWHGDPEAMRTIKAVAREYWASATA