Protein Info for ABID97_RS12155 in Variovorax sp. OAS795

Annotation: glycogen debranching protein GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02100: glycogen debranching enzyme GlgX
  • amino acids 15 to 683 (669 residues), 1060.7 bits, E=0
• PF02922: CBM_48
  • amino acids 21 to 107 (87 residues), 74.5 bits, E=6.8e-25
• PF00128: Alpha-amylase
  • amino acids 202 to 308 (107 residues), 40.8 bits, E=2.2e-14

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 96% identity to vap:Vapar_1982)

MetaCyc: 55% identical to isoamylase (Sulfolobus acidocaldarius)
Isoamylase. [EC: 3.2.1.68]

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

MetaCyc Pathways

• trehalose biosynthesis V (3/3 steps found)
• starch biosynthesis (6/10 steps found)
• starch degradation II (1/9 steps found)

KEGG Metabolic Maps

• Ascorbate and aldarate metabolism
• Glycosaminoglycan degradation
• Nucleotide sugars metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.- or 3.2.1.68

Protein Sequence (720 amino acids)

>ABID97_RS12155 glycogen debranching protein GlgX (Variovorax sp. OAS795)
MTRSQRNSITAVWPGRPYPRGANWDGEGVNFALFSQHAQAVDLCLFDDKGRHEVQRIPIR
ERTDGVWHCYLPEARPGQAYGYRVHGPYKPEEGHRFNAHKLLVDPYAKDLVGDLRWGDAL
YGYTVGSKREDLSFDRRDSAPLVPKGRVLETAFTWGDDHRPSVPWQDMVIYELHVRGFTM
RHPEVPPELRGTYGGLCCAPVVDYLKRLGVTTIELLPVHSFLNDRHLAEKGLQNYWGYNS
LAYFAPEMRYSASGKVKEFKTMVKTLHSAGIEVILDVVYNHTCEGNQLGPTLSMRGVDNA
SYYIVNNENRRYYDDFTGCGNTVNLEHPHALQLVMDSLRYWAEEMHVDGFRFDLASALAR
EAGKVENLGGFFDAIRQDPTLNRVKLIAEPWDLGHGGYQVGNFPLGWAEWNDRYRDGMRG
FWKGDAGVIGEVGKRLTGSEDLYGWSGKRPNASINFITAHDGFTLDDLVSYNDKHNEANG
EDNRDGNSHNISWNCGAEGPSDDPEIAGLRERQKRNLLATLLLSQGVPMLLAGDERGHTQ
NGNNNAYCQDNDISWIDWTPTPERQALVTFVERTIALRRAHPSFRRRSFFAGKPTEADSV
TDVVWLRPDGAEMRPEDWSDGNARCLAMYMSGGGIVDRGPRGEAQHDDDFLVLFNAHHDE
IRFTLPPARYGTWRLLLDTASGTPPPATEDVAALAPAWSEPAYPLQCRSLVVLSRPDVRP