Protein Info for ABID97_RS11550 in Variovorax sp. OAS795
Annotation: alpha-hydroxy acid oxidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K15054, (S)-mandelate dehydrogenase [EC: 1.1.99.31] (inferred from 63% identity to vap:Vapar_3912)MetaCyc: 40% identical to sulfolactate dehydrogenase (Roseovarius nubinhibens ISM)
1.1.1.-
Predicted SEED Role
"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)
MetaCyc Pathways
- mandelate degradation to acetyl-CoA (14/18 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (25/35 steps found)
- methylglyoxal degradation V (2/3 steps found)
- sulfolactate degradation III (2/3 steps found)
- mandelate degradation I (3/5 steps found)
- sulfolactate degradation II (2/4 steps found)
- superpathway of sulfolactate degradation (3/6 steps found)
- lactate fermentation to acetate, CO2 and hydrogen (Desulfovibrionales) (3/8 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (22/42 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.2.3, 1.1.99.31
Use Curated BLAST to search for 1.1.2.3 or 1.1.99.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (391 amino acids)
>ABID97_RS11550 alpha-hydroxy acid oxidase (Variovorax sp. OAS795) MKLNANDHRRHARRRLPRFVFDYVDGGAENEDCLRRNTMDLAGLHLLPTALRDTRHADTD ITVFGRRWSAPTGVAPIGFSGLVRPHGDTLLACAAASVGVPHVLPTPSNDRLEAVREAAL ARNPDAVQWMQLYVMGDRTIAEQLVRRARHAGYGALVLTVDATVSGLRERDVRNGFKLPF RFTLSTLLDLALHPSWSLAMAMQGRSPSFVNLTESDDGATSPKVQAALLSRTMDRTLVWE HLAWLRRLWDGPLVIKGLLHPHDAERAIAHGADAIVVSNHGGRQLDAAPSTISVLPRMVD AVAQRIPVFVDGGFRRGSDVVKALAAGATAAFVGRPAAWGMATEGEAGARSVLAALSEEI ARTMILIGADRVADISAQHLLANPQAGDRRG