Protein Info for ABID97_RS11115 in Variovorax sp. OAS795

Annotation: isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01346: isocitrate lyase
  • amino acids 16 to 260 (245 residues), 364.1 bits, E=5.2e-113
  • amino acids 261 to 438 (178 residues), 290.3 bits, E=1.2e-90
• PF00463: ICL
  • amino acids 20 to 260 (241 residues), 200.7 bits, E=3.9e-63
  • amino acids 261 to 438 (178 residues), 171.5 bits, E=2.8e-54
• PF13714: PEP_mutase
  • amino acids 86 to 292 (207 residues), 56.3 bits, E=3.6e-19

Best Hits

Swiss-Prot: 75% identical to ACEA_ECOLI: Isocitrate lyase (aceA) from Escherichia coli (strain K12)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 98% identity to vap:Vapar_1869)

MetaCyc: 75% identical to isocitrate lyase (Escherichia coli K-12 substr. MG1655)
Isocitrate lyase. [EC: 4.1.3.1]

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.1

Protein Sequence (439 amino acids)

>ABID97_RS11115 isocitrate lyase (Variovorax sp. OAS795)
MPQTLTEQLSREQQIAALEKEWATNPRWKGIKRGYSAADVVRLRGSFPIEHTLARRGAEK
LWELVNNEPYVNCLGALTGGQAMQQVKAGVKAIYLSGWQVAADANTYSAMYPDQSLYPVD
SVPTVVERINNTFRRADEIQWSKNVNPGDKGYVDYFAPIVADAEAGFGGVLNGFELMKAM
IKAGAGGVHFEDQLASVKKCGHMGGKVLVPTTEAVQKLVAARMAADVCGTPTLVIARTDA
EAADLITSDYDENDKPFLTGERTAEGFYKTRKGMDQAISRAVAYAYYADLVWCETGTPDL
EFARKFAEAVHKVHPGKMLAYNCSPSFNWKKNLDDATIAKFQKELGAMGYKYQFITLAGI
HSMWFNMFDLAQDYVQRGMSAYVEKVQEPEFAARDRGYTFVSHQQEVGTGYFDEVTTVIQ
GGKSSVTALTGSTEEEQFH