Protein Info for ABID97_RS10540 in Variovorax sp. OAS795

Annotation: DNA primase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 658 TIGR01391: DNA primase" amino acids 3 to 421 (419 residues), 452.1 bits, see alignment E=8.7e-140 PF01807: zf-CHC2" amino acids 4 to 98 (95 residues), 128.8 bits, see alignment E=1.7e-41 PF08275: DNAG_N" amino acids 129 to 255 (127 residues), 126.7 bits, see alignment E=1.8e-40 PF13662: Toprim_4" amino acids 263 to 336 (74 residues), 58 bits, see alignment E=2.8e-19 PF01751: Toprim" amino acids 265 to 336 (72 residues), 38.4 bits, see alignment E=3.4e-13 PF13155: Toprim_2" amino acids 266 to 353 (88 residues), 61.2 bits, see alignment E=3.1e-20 PF10410: DnaB_bind" amino acids 372 to 425 (54 residues), 42.7 bits, see alignment 1.6e-14

Best Hits

KEGG orthology group: K02316, DNA primase [EC: 2.7.7.-] (inferred from 94% identity to vap:Vapar_1763)

Predicted SEED Role

"DNA primase (EC 2.7.7.-)" in subsystem DNA-replication or Macromolecular synthesis operon (EC 2.7.7.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (658 amino acids)

>ABID97_RS10540 DNA primase (Variovorax sp. OAS795)
MTIPASFIQELIARADVVEIVGRYVQLKKAGANFMGLCPFHGEKSPSFSVSPTKQFYHCF
GCGVHGNAIGFLMEHAGMGFVEAVHDLAGQYGLQVPEDDASPAERARAANQRQKQATLTD
VLEKAGEAYRKALRQAPGAIEYLKGRGVSGEVAKQFGIGYAPAGWRTLASVFPDYDDPLL
AESGLVIVNTEDGAEDAKRYDRFRDRVMFPIRNVKGECIGFGGRVLGDEKPKYLNSPETP
VFSKGRELYGLYEARAAFRERGYALVTEGYMDVVALAQLGFPNAVATLGTACTTEHVQKL
FRFTESVVFSFDGDAAGRRAARKALDGALPYATDVRSIKFLFLPAEHDPDSFIREFGADA
FARFVAEATPLSRFMIEAARDGCDLTTAEGRAHMSSNVRPLWSAMPDGALKRQLLSEIAT
LVQLDAAALSELWAATPGPRKAAPAPAARHGGEPGDPFDYADMPPPSEYEPPRYENDSKP
RGKFIKGKRWGGKFEAPIEPLRGRGKPPSRPDVAVRLLLSNMAQWDALSHELHVMLCDLP
GPHGALFTWLDSQLHEHGVQPWAALREGMRGLDFEALAERLMSVAESAPPAEGEEDQHLA
DAARELSSVLDFMLDDLLKAQQSEAIAAVGSDPKALERYKALEARRLELRARLKPSGS