Protein Info for ABID97_RS08710 in Variovorax sp. OAS795

Annotation: polynucleotide adenylyltransferase PcnB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 TIGR01942: poly(A) polymerase" amino acids 30 to 470 (441 residues), 443 bits, see alignment E=5.4e-137 PF01743: PolyA_pol" amino acids 59 to 206 (148 residues), 112.9 bits, see alignment E=2.1e-36 PF12627: PolyA_pol_RNAbd" amino acids 238 to 298 (61 residues), 59.3 bits, see alignment E=3.7e-20 PF12626: PolyA_pol_arg_C" amino acids 357 to 470 (114 residues), 127.7 bits, see alignment E=3.6e-41

Best Hits

KEGG orthology group: K00970, poly(A) polymerase [EC: 2.7.7.19] (inferred from 90% identity to vap:Vapar_1467)

Predicted SEED Role

"Poly(A) polymerase (EC 2.7.7.19)" in subsystem Polyadenylation bacterial (EC 2.7.7.19)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (546 amino acids)

>ABID97_RS08710 polynucleotide adenylyltransferase PcnB (Variovorax sp. OAS795)
MIKKFIDKLLGKSAGGTQGKSRFGKRQEVPAEVHRIDPALVDERAKNVVTTLQQAGYEAY
VVGGAVRDLLLGLRPKDFDVATNATPEQVKSLFRRAFIIGRRFRIVHVVYGRGREHEVIE
VSTFRAYMDNAAAEQVKGNERTSKGELAGMTHAVDSTGRVLRDNVWGPQDEDAARRDFTV
NAMYYDPANQIVVDYHNGIKDAQKLTLRMIGDPATRYREDPVRIIRAIRFSAKLAALGFK
METKTAAPLVESSKLLADVPQSRLFDEMLKLLQTGHAIATVEQLRKLGLVTGIYPLLDVV
VERADSPFVKAALQDTDRRVGEGKPVAPSFLLACVLWADVRDGWAQRIEGRHGQRAQPPF
PALQDAIDDVFNARIGDVSGRGKLAADMREIWMMQPRFDKRTGSTPYSLVEQARFRAAFD
FMRLRADVGEVGEAIAEWWQEFSTADDVRRQDLLEQVRDEQKTRQRVRTRDPVPSKPRDE
LPGRQRQDRQDADEPAQPDDELETETAGAAGDGEAAAPRKRRRRRKPRAGGGGGGAGEGG
GSAAGE