Protein Info for ABID97_RS07515 in Variovorax sp. OAS795

Annotation: formate dehydrogenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 957 PF13510: Fer2_4" amino acids 13 to 89 (77 residues), 71.4 bits, see alignment E=3.2e-23 PF22117: Fer4_Nqo3" amino acids 158 to 223 (66 residues), 27.4 bits, see alignment 1.9e-09 PF13237: Fer4_10" amino acids 159 to 219 (61 residues), 26 bits, see alignment 4.4e-09 PF13187: Fer4_9" amino acids 164 to 222 (59 residues), 27.7 bits, see alignment 1.4e-09 PF12838: Fer4_7" amino acids 165 to 222 (58 residues), 31.5 bits, see alignment 1.2e-10 PF00037: Fer4" amino acids 206 to 223 (18 residues), 24.8 bits, see alignment (E = 8.4e-09) PF04879: Molybdop_Fe4S4" amino acids 236 to 287 (52 residues), 57.4 bits, see alignment 7e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 241 to 934 (694 residues), 719.4 bits, see alignment E=1.7e-220 PF00384: Molybdopterin" amino acids 291 to 734 (444 residues), 231.4 bits, see alignment E=9.9e-72 PF01568: Molydop_binding" amino acids 825 to 931 (107 residues), 94.2 bits, see alignment E=3.2e-30

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 81% identity to dia:Dtpsy_2797)

Predicted SEED Role

"tungsten-containing formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (957 amino acids)

>ABID97_RS07515 formate dehydrogenase subunit alpha (Variovorax sp. OAS795)
MNAPSKLAELMPQTIEFTLDGQAIQAFDGETIYKAAERHGVEIPHLCYKDGYRADGNCRA
CVVEVKGERTLAPSCCRNVTAGMDVKATSERALKSQKMVVEMLLSDMPDQGYKWIGDDAT
QQHGELSAWAKKLDIAVRPELKALRREQPKTDISHPAMAVNLDACIQCNRCVRACREEQV
NDVIGYALRGADSKIVFDLDDPMGDSTCVACGECVQACPTGALMPKSHIGSQEVDRKVDS
VCPFCGVGCLVTYNVKDEKIVSVDGRDGPANHNRLCVKGRFGFDYAHHPQRLTKPLIRKP
GMPKDFGDAPRPDDWSEYFREASWEEALALTTGKLSGLRDTYGAKSLAGFGSAKGSNEEA
YLFQKLVRTGFGSNNIDHCTRLCHASSVAALLEGVGSGAVSNQVNDVEHAGLIFVIGSNP
TANHPVAATWMKNAAQRGAKIVLADPRRTDISRHAWRTLQFKADTDVAMLNALIHAVIDE
GLVDQEFVRTRASNYEALRENVKGYSPEAMAPICGVPAETLREVARAFATAKGSMILWGM
GVSQHVHGTDNARCLIALATVTGQIGKPGSGLHPLRGQNNVQGASDAGLIPMMFPNYQRV
DNPAVHAWFEDFWGMPLDATPGYTVVEIMHKALAPDTDPHKVRGMYIMGENPAMSDPDLN
HARHALASLEHLVVQDIFMTETAWLADVVLPASAWPEKTGTVSNTDRMVQLGKRALNPPG
DAQPDLWIIQQIAKGMGLQWNYEGEESGVAAVYEEMRQAMHAVISGISWERLQRDSSVTY
PCLSEEDPGQPTVFIDDFPTADGRVKLVPADIIPADERPDAEYPFVLITGRQLEHWHTGS
MTRRATVLDALEPMATASMNQADLLKLGLEAGDVITVQSRRGEVAIHVRRDDGTPNGAVF
VPFAYYEAAANLMTNAALDPMGKIPEFKYCAVRIARGGQPMAAAGYGTGSGVLAAVD