Protein Info for ABID97_RS07510 in Variovorax sp. OAS795

Annotation: NAD(P)H-dependent oxidoreductase subunit E

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 PF01257: 2Fe-2S_thioredx" amino acids 36 to 166 (131 residues), 82.8 bits, see alignment E=4.5e-27 PF01512: Complex1_51K" amino acids 233 to 393 (161 residues), 131.4 bits, see alignment E=5.3e-42 PF10531: SLBB" amino acids 419 to 467 (49 residues), 28.6 bits, see alignment 2e-10 PF10589: NADH_4Fe-4S" amino acids 505 to 580 (76 residues), 80.6 bits, see alignment E=1.3e-26

Best Hits

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 96% identity to vap:Vapar_4073)

Predicted SEED Role

"tungsten-containing formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (581 amino acids)

>ABID97_RS07510 NAD(P)H-dependent oxidoreductase subunit E (Variovorax sp. OAS795)
MRERIRRKSKLKGRQPEEAALVEVRTLIGARPAEGHRRDLLIEHLHKLNDAFRCLHDHHL
VALAREMNIPMAEVYEVATFYHHFEVVRGGDAAPGLTVRVCDGLACELAGARDLLARLPE
LLGVDGDEVRVIAAPCVGRCEQAPVAVVDQQAVPLATTGKVLQALKSDPDQANVAYFDAA
AFAEQSVSPLPGAIERMPAFTDYATYRAHGGYTLAAALVNGEEDAEAIIKTMEDSGLRGL
GGAGFPAGRKWRIVRDQPAPRLMAVNIDEGEPGTFKDRTYLERDPHRFLEGLVVAAQIVG
IEQCYIYLRDEYHDCRALLERELARLQADPPCALPRIELRRGAGAYICGEESAMIESIEG
KRGEPRMRPPYIAQVGLFGRPTLEHNFETLYWVRDIVEKGAAWFSGFGRHGRKGLRSFSV
SGRVKHPGVKLAPAGITVQELIDEYCGGMLDGHSLYAYLPGGASGGILPARMNDIPLDFD
TLQPHGCFIGSAAVMVLSQHDRARDAALNVMRFFEHESCGQCTPCRVGTAKAAALMQAPT
WDSATLEDLAQVMGDASICGLGQAAPNPIRCIQQYFPEEVA