Protein Info for ABID97_RS07290 in Variovorax sp. OAS795

Annotation: aspartate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 TIGR00459: aspartate--tRNA ligase" amino acids 3 to 593 (591 residues), 797 bits, see alignment E=4.8e-244 PF01336: tRNA_anti-codon" amino acids 20 to 104 (85 residues), 66.6 bits, see alignment E=2.2e-22 PF00152: tRNA-synt_2" amino acids 121 to 569 (449 residues), 398.4 bits, see alignment E=3.4e-123 PF02938: GAD" amino acids 310 to 411 (102 residues), 88.3 bits, see alignment E=5e-29

Best Hits

Swiss-Prot: 98% identical to SYDND_VARPS: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 98% identity to vap:Vapar_4122)

MetaCyc: 58% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (601 amino acids)

>ABID97_RS07290 aspartate--tRNA ligase (Variovorax sp. OAS795)
MAMRTHYCGLVTEALMGQTVTLCGWVNRRRDHGGVIFVDVRDREGYVQVVCDPDRAATFA
VAENLRNEFCVQITGLVRSRPEGTTNDQLKSGKIEVLCHELKVLNPSVTPPFLLDDDNLS
ETTRLTHRVLDLRRPVMQRNMMLRYKVTMETRKFLDANGFIDIETPMLGKSTPEGARDYL
VPSRVHDGSFFALPQSPQLFKQLLMVAGYDRYYQIVKCFRDEDLRADRQPEFTQIDIETS
FLAEEEIREMFEGMIRTVFRNAAGIDLPVFPTMSYADAMFKYGSDKPDLRVKLEFTELTE
LMKRVDFKVFSNAATMQGGRVVALRVPGGGAEGGLSRGEIDAYQEFVKIYGAKGLAYIKV
NDAAAGREGLQSPIVKNLDDASLAEIIARTGARNGDILFFGADKEKVVNDAIGALRVKIG
HSAFGKKSGLFEDRWAPLWVVDFPMFEFDEEGQRWSAVHHPFTAPKDGHEDLMDTAPEKC
IAKAYDMVLNGIEMGGGSVRIHREEVQSKVFRALKINAEDAQLKFGFLLDALQYGAPPHG
GIAIGLDRLVMLMTGAESIRDVIAFPKTQRAQDLLTQAPSPVDEKQLRELHIRLRNVQQP
A