Protein Info for ABID97_RS07195 in Variovorax sp. OAS795

Annotation: glutamyl-tRNA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 transmembrane" amino acids 248 to 265 (18 residues), see Phobius details TIGR01035: glutamyl-tRNA reductase" amino acids 5 to 418 (414 residues), 392.1 bits, see alignment E=1.5e-121 PF05201: GlutR_N" amino acids 6 to 159 (154 residues), 168.4 bits, see alignment E=1.5e-53 PF01488: Shikimate_DH" amino acids 175 to 309 (135 residues), 150.6 bits, see alignment E=4.5e-48 PF00745: GlutR_dimer" amino acids 323 to 418 (96 residues), 78.1 bits, see alignment E=8.7e-26

Best Hits

Swiss-Prot: 84% identical to HEM1_POLSJ: Glutamyl-tRNA reductase (hemA) from Polaromonas sp. (strain JS666 / ATCC BAA-500)

KEGG orthology group: K02492, glutamyl-tRNA reductase [EC: 1.2.1.70] (inferred from 96% identity to vap:Vapar_4205)

MetaCyc: 44% identical to glutamyl-tRNA reductase (Escherichia coli K-12 substr. MG1655)
Glutamyl-tRNA reductase. [EC: 1.2.1.70]

Predicted SEED Role

"Glutamyl-tRNA reductase (EC 1.2.1.70)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis (EC 1.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.70

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (423 amino acids)

>ABID97_RS07195 glutamyl-tRNA reductase (Variovorax sp. OAS795)
MSVWALGLNHTTAPLDLRGRFAFALDQLAPTLHSLRNSFPTGRHPQVEAAIISTCNRTEI
YCAAEHAALEHTVGWLAESGGVAPALLRSHAYTLQDDEAARHVFRVASGLDSMVLGEAQI
LGQMKDAVRAAETAGALGSTLNQLFQRSFAVAKEVRTATEIGAHSISMAAAAVRLAGQLF
EDLHQTRVLFVGAGEMIDLAATHFAAKDPKSIAIANRTLERGEKLASRFGGEAMRLADLP
NRLAEFDIVVSCTASTLPIIGLGAVERALKTRKHRPMFMVDLAVPRDIELEVKALEDIYL
YTVDDLAQVVQQGQANRQAAVAQAEVIIDAGVQSFMHWLGQRGTVPLIQQLNAQTDEWRA
AEIARARKLLAKGESVDAVLEAMARGLTQKMLHGALAELHAGDATSREHTAQTISRLFLR
KER