Protein Info for ABID97_RS07185 in Variovorax sp. OAS795

Annotation: peptide chain release factor N(5)-glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00536: methyltransferase, HemK family
  • amino acids 13 to 282 (270 residues), 232.9 bits, E=4.2e-73
• PF17827: PrmC_N
  • amino acids 17 to 81 (65 residues), 45.9 bits, E=2.1e-15
• TIGR03534: protein-(glutamine-N5) methyltransferase, release factor-specific
  • amino acids 28 to 275 (248 residues), 268.7 bits, E=5e-84
• PF05175: MTS
  • amino acids 111 to 196 (86 residues), 51 bits, E=4.2e-17
• PF06325: PrmA
  • amino acids 111 to 164 (54 residues), 24.2 bits, E=6.4e-09
• PF13847: Methyltransf_31
  • amino acids 121 to 178 (58 residues), 31.2 bits, E=5.1e-11
• PF13649: Methyltransf_25
  • amino acids 122 to 178 (57 residues), 33.7 bits, E=1.5e-11
• PF08241: Methyltransf_11
  • amino acids 123 to 173 (51 residues), 21.9 bits, 6.9e-08

Best Hits

Swiss-Prot: 54% identical to PRMC_XANCP: Release factor glutamine methyltransferase (prmC) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K02493, methyltransferase [EC: 2.1.1.-] (inferred from 86% identity to vap:Vapar_4207)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmC, methylates polypeptide chain release factors RF1 and RF2"

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Protein Sequence (284 amino acids)

>ABID97_RS07185 peptide chain release factor N(5)-glutamine methyltransferase (Variovorax sp. OAS795)
MTPTAPTAPATVAQALAAAVARGVDRLDAQLLLLHALGRAPHDRAWLLAHDTDAIAGTAW
ASLSAQLSRRLAGEPVAYLLGEKEFHGLRLHVDARVLVPRPDTETLVDWALQCLEGREKP
RVLDLGTGSGAIALALQHARADARVDAVDASADALAVAKANAQRLRLPVGFSQAHWLDGA
ASGYAVIASNPPYIAAGDPHLPALRHEPASALVAGADGLDDIRQIVRDGPSHLAEGGWLL
LEHGHDQAPAVRRLLEARGFAEVQSRVDLAGIQRCSGGVWRTVK