Protein Info for ABID97_RS05930 in Variovorax sp. OAS795

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 57% identical to ASPA_HAEIN: Aspartate ammonia-lyase (aspA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 97% identity to vap:Vapar_0827)

MetaCyc: 54% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

L-glutamate degradation II (2/2 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
L-aspartate degradation II (aerobic) (2/3 steps found)
L-aspartate degradation III (anaerobic) (2/3 steps found)
L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.1

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (483 amino acids)

>ABID97_RS05930 aspartate ammonia-lyase (Variovorax sp. OAS795)
MTANFRTEHDFLGEKQIPAMAYWGVHTARAVENFAISGTRISAMPDLVRALAFVKKAATR
ANADLGAIDRDRAAAIVLACEDLIEGKLLDEFVVDVIQGGAGTSTNMNANEVICNLALEK
LGHEKGRYDVLHPNDHVNASQSTNDVYPTAVRLALWFAIGRLLEAMAALRKSFEAKALEF
KDILKIGRTQLQDAVPMTLGQEFLTYAIMIGEDEARLGEARALIEEINLGATAIGTGINA
PHGYANLACQYLAEQTGVPLKQSANLIEATQDTGAFVQLSGVLKRVATKLSKTCNDLRLL
SSGPQAGFGEIRLPARQAGSSIMPGKVNPVIPEVMNQVAFEVIGNDITVTMASEAGQLQL
NAFEPIMGWSLFKSIQHLGNACTTLRQNCVDGIEANREFLAKRVRESVTLVTALNPLIGY
EKAALIAKTALATGGPIDLVAESLGIMTRAEMEALLVPENLTQPVRLSAPVPPAAEPSGT
KAA