Protein Info for ABID97_RS05425 in Variovorax sp. OAS795
Annotation: SMP-30/gluconolactonase/LRE family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01053, gluconolactonase [EC: 3.1.1.17] (inferred from 90% identity to vap:Vapar_0705)Predicted SEED Role
"Gluconolactonase (EC 3.1.1.17)" in subsystem Entner-Doudoroff Pathway (EC 3.1.1.17)
MetaCyc Pathways
- sorbitol biosynthesis II (3/3 steps found)
- glucose and glucose-1-phosphate degradation (4/5 steps found)
- glucose degradation (oxidative) (4/5 steps found)
- Entner-Doudoroff pathway III (semi-phosphorylative) (6/9 steps found)
- L-ascorbate biosynthesis IV (animals, D-glucuronate pathway) (3/6 steps found)
- L-ascorbate biosynthesis VI (plants, myo-inositol pathway) (1/4 steps found)
- Entner-Doudoroff pathway II (non-phosphorylative) (4/9 steps found)
- L-ascorbate biosynthesis VIII (engineered pathway) (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.17
Use Curated BLAST to search for 3.1.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (367 amino acids)
>ABID97_RS05425 SMP-30/gluconolactonase/LRE family protein (Variovorax sp. OAS795) MQNGSRRRRQFLKTAAGSGLAALAGVADAQSFEFRPNQRYPDPSVLILDPSFAKYRIYSS TVEQVGTGMRWAEGPVYFPEGGYLLCSDIPNNRIMKYDEKTGRFTVHRQNANFANGNTRD RQGRLVTCEHSVTRRVVRTEKDGRMTVLADSFEGKKLNAPNDVVVRSDDTVWFTDPTFGI NGEWEGSRATPEQATTNVYRIATDGKLTAVITDLVNPNGLAFSPDEKKLYVVEWKGTPHR SIWSYDVSADGASVSNKTKLIDADGPGALDGFRVDRDGNLWCGWGFSGAFAPEATDIGGG MKAHLPLGKSEDFDGVKVFNPQGKPIGFIRLPERCANLEFGGPKRNRLYMASSHSLYALY VEAHGAV