Protein Info for ABID97_RS04715 in Variovorax sp. OAS795

Annotation: haloacid dehalogenase type II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 TIGR01428: haloacid dehalogenase, type II" amino acids 6 to 201 (196 residues), 215.2 bits, see alignment E=9e-68 PF00702: Hydrolase" amino acids 6 to 187 (182 residues), 76.1 bits, see alignment E=5.2e-25 TIGR01493: HAD hydrolase, family IA, variant 2" amino acids 6 to 188 (183 residues), 88.8 bits, see alignment E=4.9e-29 PF13419: HAD_2" amino acids 95 to 197 (103 residues), 40 bits, see alignment E=4.6e-14

Best Hits

Swiss-Prot: 44% identical to HAD_AGRTR: 2-haloalkanoic acid dehalogenase from Agrobacterium tumefaciens (strain RS5)

KEGG orthology group: K01560, 2-haloacid dehalogenase [EC: 3.8.1.2] (inferred from 90% identity to vap:Vapar_0453)

Predicted SEED Role

"(S)-2-haloacid dehalogenase (EC 3.8.1.2)" (EC 3.8.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.8.1.2

Use Curated BLAST to search for 3.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (225 amino acids)

>ABID97_RS04715 haloacid dehalogenase type II (Variovorax sp. OAS795)
MDHPGAVLFDAYGTLFDVYSVAETAERLFAGQGQALAAGWRDKQIEYTRLVTTSDDGAHY
QPFGELTRAALRYTCKRLGLGLTEAAEQQLMGQYRTLTAFPECREVLQALKRRGVVTGIL
SNGDPGMLDAAVQSAGLEGLLDHVLSVDGIRKYKTHPEAYRLGLTATGLAPAQIAFVSCN
GWDALGATWYGFRTLWVNRYQLPFEELGTSPERTGTSLRDVLGFF