Protein Info for ABID97_RS04500 in Variovorax sp. OAS795

Annotation: HPr(Ser) kinase/phosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 PF02603: Hpr_kinase_N" amino acids 8 to 135 (128 residues), 82.8 bits, see alignment E=2.2e-27 TIGR00679: HPr(Ser) kinase/phosphatase" amino acids 15 to 312 (298 residues), 316.4 bits, see alignment E=9.5e-99 PF07475: Hpr_kinase_C" amino acids 138 to 307 (170 residues), 203.4 bits, see alignment E=2e-64

Best Hits

Swiss-Prot: 98% identical to HPRK_VARPS: HPr kinase/phosphorylase (hprK) from Variovorax paradoxus (strain S110)

KEGG orthology group: K06023, HPr kinase/phosphorylase [EC: 2.7.11.- 2.7.4.-] (inferred from 98% identity to vap:Vapar_0405)

Predicted SEED Role

"HPr kinase/phosphorylase (EC 2.7.1.-) (EC 2.7.4.-)" in subsystem Mannitol Utilization (EC 2.7.1.-, EC 2.7.4.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.-, 2.7.4.-

Use Curated BLAST to search for 2.7.1.- or 2.7.11.- or 2.7.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (319 amino acids)

>ABID97_RS04500 HPr(Ser) kinase/phosphatase (Variovorax sp. OAS795)
MKPTVISADAMFEEFRGSLRWEWLAGLGASERQFDPEVISRAQSAADLVGYLNYIHPYRV
QILGAREVSYLTRGSPEDCARRIARIVTLEPPMLVLADGQAAPDELLSICERAQLPLFAT
RESSAYVIDLLRAYLSKHFAERTSMHGVFMDILGMGVMITGESGLGKSELGLELISRGNG
LVADDAVDLYRINQNTIEGRCPDLLLNLLEVRGIGLLDIRAIFGETAVRRKMRLKLIVHL
VRRDSFERDYERMPSAPLTQDVLGIPVRKVIIQVVAGRNIAVLVEAAVRNSILQLRGIDT
YADFVARHHKAMENGRDGD