Protein Info for ABID97_RS03450 in Variovorax sp. OAS795

Annotation: homocysteine S-methyltransferase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02574: S-methyl_trans
  • amino acids 22 to 330 (309 residues), 323.8 bits, E=7.4e-101

Best Hits

KEGG orthology group: K00548, 5-methyltetrahydrofolate--homocysteine methyltransferase [EC: 2.1.1.13] (inferred from 98% identity to vap:Vapar_0167)

Predicted SEED Role

"5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)" in subsystem Methionine Biosynthesis (EC 2.1.1.13)

MetaCyc Pathways

• aspartate superpathway (24/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (17/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (8/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (8/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (7/8 steps found)
• L-methionine biosynthesis III (4/4 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• L-methionine biosynthesis I (4/5 steps found)
• folate transformations II (plants) (8/11 steps found)
• L-methionine salvage from L-homocysteine (1/3 steps found)
• folate transformations I (7/13 steps found)
• superpathway of L-methionine salvage and degradation (8/16 steps found)

KEGG Metabolic Maps

• Methionine metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.13

Use Curated BLAST to search for 2.1.1.13

Protein Sequence (348 amino acids)

>ABID97_RS03450 homocysteine S-methyltransferase family protein (Variovorax sp. OAS795)
MKPVIYTRGQALAGILEKRIAILDGAMGTMIQRFKLTEEQYRGERFKDFERDVKGNNELL
SLTRPDVIRDIHEGYLGAGADLIETNTFGATTIAQEDYKMAHLAREMNLESAKLARAACD
KFSTPDKPRFVAGALGPTPKTASISPDVNDPGARNVDFEQLRAAYYEQTEALVEGGADVI
LVETIFDTLNAKAALFAVDEYFDNSGQRLPLIISGTVTDASGRILSGQTVTAFWHSVRHA
QPLAIGLNCALGAALMRPYIQELAKVAGDTFISCYPNAGLPNPMSETGFDETPDVTSRLL
HEFAAEGLVNIVGGCCGTTPDHIAAIGQAVAPLAGRMLNSNGFYREAA