Protein Info for ABID97_RS01810 in Variovorax sp. OAS795

Annotation: methionyl-tRNA formyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 321 transmembrane" amino acids 275 to 291 (17 residues), see Phobius details TIGR00460: methionyl-tRNA formyltransferase" amino acids 4 to 312 (309 residues), 269.2 bits, see alignment E=2.2e-84 PF00551: Formyl_trans_N" amino acids 5 to 190 (186 residues), 146.2 bits, see alignment E=9.5e-47 PF02911: Formyl_trans_C" amino acids 211 to 312 (102 residues), 90.3 bits, see alignment E=8.2e-30

Best Hits

Swiss-Prot: 91% identical to FMT_VARPS: Methionyl-tRNA formyltransferase (fmt) from Variovorax paradoxus (strain S110)

KEGG orthology group: K00604, methionyl-tRNA formyltransferase [EC: 2.1.2.9] (inferred from 91% identity to vap:Vapar_5111)

Predicted SEED Role

"Methionyl-tRNA formyltransferase (EC 2.1.2.9)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or Folate Biosynthesis (EC 2.1.2.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.9

Use Curated BLAST to search for 2.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (321 amino acids)

>ABID97_RS01810 methionyl-tRNA formyltransferase (Variovorax sp. OAS795)
MNPLRVVFAGTPEFARVALEAIAAAGHDIALVLSQPDRPAGRGMKLQASPVKQCAVAHGW
PVAQPRSLRLDGKYPEDAAAARAALAAARPDVMVVAAYGLILPQWVLDLPASGCLNIHAS
LLPRWRGAAPIHRAIEAGDAQTGITIMQMDAGLDTGDMLLREAVDIGGDNTARLHDRLAE
LGGRMIVHALARVGQLARTPQPAEGVTYASKVEKHEAQIDWNQPAEAIVRRIRAFDPFPG
ANSPLDGETVKLWSAHAVPAEVPAEVSAAPGTLLAVADTGVAVAAAGSIVMVTELQRPGG
KRLAVADFLRGFDLKPGQRFG