Protein Info for ABID97_RS01595 in Variovorax sp. OAS795

Annotation: tRNA guanosine(34) transglycosylase Tgt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 TIGR00449: tRNA-guanine family transglycosylase" amino acids 12 to 383 (372 residues), 475.8 bits, see alignment E=8.1e-147 TIGR00430: tRNA-guanine transglycosylase" amino acids 12 to 384 (373 residues), 504.4 bits, see alignment E=1.8e-155 PF01702: TGT" amino acids 14 to 386 (373 residues), 519.6 bits, see alignment E=2.3e-160

Best Hits

Swiss-Prot: 87% identical to TGT_DELAS: Queuine tRNA-ribosyltransferase (tgt) from Delftia acidovorans (strain DSM 14801 / SPH-1)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 96% identity to vap:Vapar_5071)

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>ABID97_RS01595 tRNA guanosine(34) transglycosylase Tgt (Variovorax sp. OAS795)
MLNFELLKADPASHARRGTLTLNHGVVQTPIFMPVGTYGTVKGVMPRSLEEMGAQIILGN
TFHLWMRPGLDVMASFGGLHQFEKWNKPILTDSGGFQVWSLGAMRKISEEGVKFASPVNG
DKLFLTPEVSMQIQTILNSDIVMQFDECTPYDTKGHITTEAEARISMELSLRWARRCQHE
FARLENPNALFGIVQGGMFENLREESLAALVEMDFPGYAIGGVSVGEPKEEMLHIMAHTP
HRLPAHKPRYLMGVGTPEDLVQGVADGVDMFDCVMPTRNARNGTLFTRFGDLKMRNARHK
NDPQPIDPSCTCHACAGTSGVSWNDGGREGFSRAYLHHLDRCAEMLGPMLTTIHNLHYYL
NLMREIREALEADSFTSFRARFKADRARGV