Protein Info for ABID97_RS01155 in Variovorax sp. OAS795

Annotation: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 814 transmembrane" amino acids 14 to 37 (24 residues), see Phobius details amino acids 44 to 61 (18 residues), see Phobius details amino acids 68 to 84 (17 residues), see Phobius details amino acids 93 to 116 (24 residues), see Phobius details amino acids 128 to 146 (19 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 18 to 812 (795 residues), 1074.5 bits, see alignment E=0 PF01011: PQQ" amino acids 240 to 267 (28 residues), 22.8 bits, see alignment (E = 6e-09) amino acids 465 to 492 (28 residues), 27.6 bits, see alignment (E = 1.8e-10) amino acids 686 to 720 (35 residues), 26.3 bits, see alignment (E = 4.4e-10) amino acids 743 to 777 (35 residues), 23 bits, see alignment (E = 4.9e-09) PF13360: PQQ_2" amino acids 248 to 480 (233 residues), 32.1 bits, see alignment E=9.4e-12 amino acids 693 to 794 (102 residues), 27 bits, see alignment E=3.4e-10

Best Hits

Swiss-Prot: 48% identical to DHG_ECOLI: Quinoprotein glucose dehydrogenase (gcd) from Escherichia coli (strain K12)

KEGG orthology group: K00117, quinoprotein glucose dehydrogenase [EC: 1.1.5.2] (inferred from 89% identity to vap:Vapar_5020)

MetaCyc: 48% identical to quinoprotein glucose dehydrogenase (Escherichia coli K-12 substr. MG1655)
Quinoprotein glucose dehydrogenase. [EC: 1.1.5.2]

Predicted SEED Role

"Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2)" in subsystem D-gluconate and ketogluconates metabolism or Entner-Doudoroff Pathway or Respiratory dehydrogenases 1 (EC 1.1.5.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (814 amino acids)

>ABID97_RS01155 membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family (Variovorax sp. OAS795)
MPDAAVRVRAPSSTLLTVTAAVIGLAGLCLGAAGAWLAVLGGSWYYLAAGLGLMLAAVLL
PRGGGAGLWAYALVVAFTLAWALWEVGFDWWPLAARGDVFFVIGLFLLTPWVARALSRRH
PRGVAPARAFLGVVLLAFLLAAVVSWTRDPTRVEGNAPASAAPAAAAEASAAGSPAPPKD
DWTAYGGNGMGQRYSALDQITPANVGQLEEAWHFRTGDVRGRRGDPEETTFEVTPLKIGD
TLFLCTPHQSVVALNATTGTQLWRYTPQIRQPLALQHLTCRGLSYDPGSAVRPAAASASR
PSVPAPGDCDAKLFMPTADGRVIALDPGSGKPCMRFGGGSGQIDLWQNMPNVRPGSYYST
SPVVVTRSLIIVGGTVLDNVSTQETSGVIRAFDIDTGALVWNWDAGNPGDTAPIAPGRTY
TANSPNSWSISSVDEALGMVYVPTGNQPPDQWGGKRSEGAERYSSSVVALDLATGRVRWS
FQTVHHDLWDYDVPSQPSLVDLRVGNETVPALVQPTKQGELFVLDRRDGKPVVKVHERPA
PQDAAAGDHASLTQPVSELSFDPPALRPADMWGGTIFDQLACRIAFHRLRYEGRYTPPST
RGSLIYPGNFGVFNWGSIAVDPQRQVAFTTPASLAFVSRLVPRKDDTSLYVQGKDRPGDS
LPALNENFGAPFAVQLSAFTSVFGLPCQAPPWGYVAGADLRTGRVLWKHKNGTVRDSSPL
PLPFAMGVPNLGGPVMTAGGVAFLSGTLDYFVRAYDVADGKELWRSRLPAGGQATPMTYR
GSDGRQYVVVAAGGHGSLGTRTGDHVIAYALPRR