Protein Info for ABID97_RS00240 in Variovorax sp. OAS795

Annotation: L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 990 PF14850: Pro_dh-DNA_bdg" amino acids 51 to 153 (103 residues), 89.8 bits, see alignment E=2.7e-29 PF01619: Pro_dh" amino acids 168 to 461 (294 residues), 325.9 bits, see alignment E=3.8e-101 TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase" amino acids 525 to 968 (444 residues), 548.4 bits, see alignment E=7.1e-169 PF00171: Aldedh" amino acids 527 to 972 (446 residues), 350.9 bits, see alignment E=1.5e-108

Best Hits

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 92% identity to vap:Vapar_4810)

Predicted SEED Role

"Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.12

Use Curated BLAST to search for 1.5.1.12 or 1.5.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (990 amino acids)

>ABID97_RS00240 L-glutamate gamma-semialdehyde dehydrogenase (Variovorax sp. OAS795)
MHLPIPYRPEAEVVSHRLASLEGALDWSAAAATARPWVEAVRRHPPPFWAMESLLREYPI
SSAEGLALMRLAEALLRVPDAATAIALTADQLGRADFDGAADSTLSRLSSAAIAMSKKFL
PEGDHPPGLMAKLGARTVVAATLRAVQLLGRQFVLGQTIAEAMDEARSAHRRHDALRFSY
DMLGEGARTDADALRYLDSYTHAIRAIATGADAARAPEHNDGISIKLSALHPRYEEAQRE
RVMRELVPRVWQLCELAAAANLNLTIDAEEVDRLELSLDVFEALAARVAAGQPQWRGFGL
ALQAYQTRSLELIVHVTAVARRHGLRLMCRLVKGAYWDAEIKRAQELGLPHYPVFTHKHH
SDISYLACARALLSAPDAVYPQFATHNAGTIAAILQMAEAAGAPFELQRLHGMGEGVYRE
VMKRTAAPVRVYAPVGQHKDLLAYLVRRLLENGANSSFVNQLGDDSVDIEELLTSPLWLE
PTNPALPLPPELFGPAPARRNSEGVDLAVESMRAPLLGALATTHVPAVEEFKAGEALKAV
AASAAAFRSWRKTPVETRAAMLRQAADALQRELPRFCALLVKEAFKTWGDAVAEVREAID
FLRYYADEAERIMQPVALPGPTGESNELRLTARGPWVCISPWNFPLAIFMGQVAAALATG
NTVLAKPAEQTPAVALEAVKLLHAAGVPADAVQLLHGPGDTVGAALVAAPGVAGVVFTGS
TQVARIIHRALAAKDGPIVPLIAETGGINAMLVDSSALPEQVVDAVVQSAFRSAGQRCSA
LRLLVLHDSIADAVIEMIRGAAGELAAGDPALLSTDVGPVIDSEAAGNIRRELARLDAEA
KPLLPSAASPAGGNLLAPQAYEVRSIDGVTSEIFGPVLQIVRWGHGALVDPADVIDRINA
LGYGLTLGIQTRIDSRAQALATRAHVGNIYINRNIIGAVVGVQPFGGEGLSGTGPKAGGP
HYLYRFCAEQTVTVNTTAAGGNAALLSAVA