Protein Info for ABI39_RS16535 in Phocaeicola dorei CL03T12C01

Annotation: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 42% identical to BIOF_THEAB: 8-amino-7-oxononanoate synthase (bioF) from Thermosipho africanus (strain TCF52B)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 100% identity to bvu:BVU_3166)

MetaCyc: 41% identical to BioF (Lysinibacillus sphaericus)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

biotin biosynthesis I (13/15 steps found)
8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
biotin biosynthesis II (4/6 steps found)
8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
8-amino-7-oxononanoate biosynthesis III (1/2 steps found)

KEGG Metabolic Maps

Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.47

Use Curated BLAST to search for 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (395 amino acids)

>ABI39_RS16535 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme (Phocaeicola dorei CL03T12C01)
MGLLQDKLSKFRLPQLYMERGVYPYFREIDSHQDTEVIMDGKKVLMFGSNSYMGLTYDER
IIEAAIAATRKYGTGCAGSRFLNGTLDLHVQLEKELAEFVGKDDALVYSTGFTVNEGVVS
CLTDRNDYIICDDRDHASIVDGRRLSFSTQLKYKHNDMEALEKELQKCKPESVKLIVVDG
VFSMEGDLANLPEIVRLKDKYNASIMVDEAHGLGVFGRDGRGVCDHFGLTDQVDVIMGTF
SKSLASIGGFVAADKDTINWLRHNSRSYIFSASNTPAATAAARAALHILKSEPERRENLW
KITNYALDCFRQAGFEIGDTESPIIPLYVRDTDKTFEVTKLAFDEGIFINPVIPPACAPQ
DTLVRVALMATHTKEQVDYAVDKLTKCFKKLEIIK