Protein Info for ABI39_RS09975 in Phocaeicola dorei CL03T12C01

Annotation: DUF1295 domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02544: Steroid_dh
  • amino acids 102 to 254 (153 residues), 140.6 bits, E=4.1e-45
• PF06966: DUF1295
  • amino acids 146 to 214 (69 residues), 28.3 bits, E=1.3e-10

Best Hits

Swiss-Prot: 36% identical to S5A1_HUMAN: 3-oxo-5-alpha-steroid 4-dehydrogenase 1 (SRD5A1) from Homo sapiens

KEGG orthology group: K12343, 3-oxo-5-alpha-steroid 4-dehydrogenase 1 [EC: 1.3.99.5] (inferred from 99% identity to bvu:BVU_1964)

MetaCyc: 36% identical to 3-oxo-5alpha-steroid 4-dehydrogenase 1 (Homo sapiens)
Cholestenone 5-alpha-reductase. [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]; 1.3.1.22 [EC: 1.3.1.22]

Predicted SEED Role

"3-oxo-5-alpha-steroid 4-dehydrogenase"

MetaCyc Pathways

• allopregnanolone biosynthesis (1/2 steps found)
• testosterone and androsterone degradation to androstendione (aerobic) (1/3 steps found)
• androgen biosynthesis (1/6 steps found)
• digitoxigenin biosynthesis (3/10 steps found)
• backdoor pathway of androgen biosynthesis (1/11 steps found)
• 11-oxyandrogens biosynthesis (4/20 steps found)
• superpathway of testosterone and androsterone degradation (2/28 steps found)
• androstenedione degradation II (anaerobic) (1/27 steps found)
• superpathway of steroid hormone biosynthesis (1/28 steps found)
• superpathway of cholesterol degradation III (oxidase) (1/49 steps found)

KEGG Metabolic Maps

• Androgen and estrogen metabolism
• C21-Steroid hormone metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.22 or 1.3.99.5

Protein Sequence (254 amino acids)

>ABI39_RS09975 DUF1295 domain-containing protein (Phocaeicola dorei CL03T12C01)
MGQQTFEFLLLAMSALAVIVFVALYYVRAGYGMFHTPKWGLSVNNKLGWVLMEAPVFLVM
LYLWWNSSVRFDAAPFLFFLLFELHYFQRSFLFPFLMKGKSRMPLAIMLMGVVFNVLNGL
MQGEWLFYLAPEGLYTDAWLSTPSFWLGIILFFIGMGINLHSDSVIRHLRKPGDTRHYLP
QKGMYRYVTSGNYFGELVEWIGFAVLTCSPAAWVFVLWTFANLAPRANSIRNRYREEFGK
DAVGKKKRMIPFIY