Protein Info for A4249_RS16140 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01368: carbamoyl-phosphate synthase, small subunit
  • amino acids 12 to 383 (372 residues), 422.9 bits, E=4.9e-131
• PF00988: CPSase_sm_chain
  • amino acids 13 to 142 (130 residues), 157.2 bits, E=2.8e-50
• PF00117: GATase
  • amino acids 203 to 381 (179 residues), 178.8 bits, E=1.4e-56
• PF07722: Peptidase_C26
  • amino acids 259 to 329 (71 residues), 22 bits, E=1.9e-08

Best Hits

Swiss-Prot: 79% identical to CARA_CAUVC: Carbamoyl-phosphate synthase small chain (carA) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01956, carbamoyl-phosphate synthase small subunit [EC: 6.3.5.5] (inferred from 90% identity to bsb:Bresu_1004)

MetaCyc: 52% identical to carbamoyl-phosphate synthetase small subunit (Escherichia coli K-12 substr. MG1655)
Carbamoyl-phosphate synthase (glutamine-hydrolyzing). [EC: 6.3.5.5]

Predicted SEED Role

"Carbamoyl-phosphate synthase small chain (EC 6.3.5.5)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 6.3.5.5)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (17/18 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• UMP biosynthesis II (6/6 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of pyrimidine ribonucleotides de novo biosynthesis (8/9 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-citrulline degradation (3/3 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• UMP biosynthesis I (5/6 steps found)
• UMP biosynthesis III (5/6 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of arginine and polyamine biosynthesis (11/17 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)
• guadinomine B biosynthesis (2/13 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.5

Use Curated BLAST to search for 6.3.5.5

Sequence Analysis Tools

See A0A168NVE2 at UniProt or InterPro

Protein Sequence (386 amino acids)

>A4249_RS16140 glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit (Brevundimonas sp. GW460-12-10-14-LB2)
MTTKILPGATGVLALADGTILQGIGVGAVGSALGEVVFNTAMTGYQEILTDPSYMSQILA
FTFPHVGNVGTNVEDIEQMGGGSDTSARGAIFRDVPTDPANYRSDASFDDWMKRRGVVGL
AGVDTRALTKIIRDKGAPHAVIAHDPDGNFDLEALVAQAKGWTGLVGLDLAKPASTLQAF
EWNEGLWDWPEGHPKTEGRKDVVVIDYGVKRNILRALASTGARITVVPATTTAEEVLARN
PDGVVLSNGPGDPAATGEYAVPEIQKLVASGKPLMGICLGHQMLALALGAKTVKMDQGHH
GANHPVKDLTTGKVEIVSMNHGFTVDRDSLPDAVTETHVSLFDGTNCGIAVKDKPIFSVQ
HHPEASPGPTDSLYLFDRFADLMGVR