Protein Info for A4249_RS16135 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: glycoside hydrolase family 3 N-terminal domain-containing protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K05349, beta-glucosidase [EC: 3.2.1.21] (inferred from 66% identity to bsb:Bresu_3081)Predicted SEED Role
"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)
MetaCyc Pathways
- neolinustatin bioactivation (2/3 steps found)
- linamarin degradation (1/2 steps found)
- lotaustralin degradation (1/2 steps found)
- linustatin bioactivation (2/4 steps found)
- cellulose degradation II (fungi) (1/3 steps found)
- coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
- α-tomatine degradation (1/6 steps found)
- firefly bioluminescence (2/14 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.21
Use Curated BLAST to search for 3.2.1.21
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160I1E6 at UniProt or InterPro
Protein Sequence (745 amino acids)
>A4249_RS16135 glycoside hydrolase family 3 N-terminal domain-containing protein (Brevundimonas sp. GW460-12-10-14-LB2) MPVSAQASRTVARHRQKIEDLIAAMTVEEKAGQLNLLADPFRWMPTAVNPLDGTGDPARV TALIREGKVGSLFNGIGAEAGRRIQRVAMEESRLKIPLLFAADVIHGLSTIFPVPLAEAA AFDTELARRTARAAAVETAASAVHQTYAPMVDVARDQRWGRNVEGAGEDVLLNNLLAAAR VRGFQGEKGLDDRDAVLATAKHMAAYSAAVGGVEYNTTDMSEQTLRGVFLPPFKASVDAG ALSIMSAFNDVNGVPASGSRKLLTDILRGEWGFEGFVVSDYTSEQELVAHGFAEDGRDAA RLAFNAGVDVSMVSGLYLEHLPSLVASGEVSMARMDEAVRRVLNTKAALGLFDDPYRGTD PVREKAVVGSREHVELSREAGRKSVVLLKNDNGLLPLKKSQKIALVGPFADDVDNVWGPW TIWGAPERRVSLEAGFRAAMSDPQNLTLARGSGVETPLDGGIEQAIAAARDAEVIVLAIG EATNMSGEAQSRTEIVVPAPQVALVDAMAALGKPMVILLRNGRALALEGNVKNAQAIVVT WFLGEQMGHAVADVLFGDHGPSARLPVSFPHKSGQQPYSYDRKTTGRPANPDLASEEYKA RYRETTNTALYPFGYGLTYGAISYAPVEMESDKLAWAGTLDLAVTVTNTGSHPAEELVQL YIHDRVASLTQPGRLLKDFKRVSLKPGQSTKVTFTLNARQLGFIGADETWRIEPGLFDVW LAPHAQGGAKATFQLIGPASITDGR