Protein Info for A4249_RS16110 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: NAD(P)-dependent oxidoreductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 76% identity to bsb:Bresu_3100)Predicted SEED Role
"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
- superpathway of L-citrulline metabolism (7/12 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160I2B7 at UniProt or InterPro
Protein Sequence (475 amino acids)
>A4249_RS16110 NAD(P)-dependent oxidoreductase (Brevundimonas sp. GW460-12-10-14-LB2) MHKTLKQGGTFVVEPRRLSKASAELRARGFGEITIPPSTETAEKQASRCTDCGVPFCQNA CPLQNNIPDWLRLSAENEPREAWRVASLTSTMPEICGRICPQDRLCEGSCTLNQSGWDAV TIGSVEAWLGDQAFSNGWVEPIRPAAERGETVGIVGAGPAGLAAADRLRCEGYQVTVYDR HDRAGGLLIYGIPGFKLEKHVVQRRVDRLIDGGVQFVLGCEVGKDVTLTELRERHDVVLL AMGVYQPRILSAPGRGPDSTVAALSYLTHQNRRDLGDAEQDGWHEARGKRVVVIGGGDTA MDCVRTAVRQGAASVTCLYRRDRENMPGSAREVVNAEEEGVVFEWLAAPKALLSQGDQVT GVRAARMQLTEGLPGQRRDIVPVPGADFDVPADIVIEALGFSPEPFAAHEADLRLRDDGT IKVGSVSFATSLPGVFAAGDAVRGASLVVWAVREGQDAAAEIDRYFKTRTEEVAA