Protein Info for A4249_RS15715 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 transmembrane" amino acids 478 to 497 (20 residues), see Phobius details PF13667: ThiC-associated" amino acids 41 to 109 (69 residues), 85.1 bits, see alignment E=2.4e-28 TIGR00190: phosphomethylpyrimidine synthase" amino acids 151 to 598 (448 residues), 706.4 bits, see alignment E=5.8e-217 PF01964: ThiC_Rad_SAM" amino acids 152 to 595 (444 residues), 620.6 bits, see alignment E=1.3e-190

Best Hits

Swiss-Prot: 82% identical to THIC_CAUSK: Phosphomethylpyrimidine synthase (thiC) from Caulobacter sp. (strain K31)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 95% identity to bsb:Bresu_3209)

MetaCyc: 82% identical to HMP-P synthase monomer (Caulobacter vibrioides)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NUG9 at UniProt or InterPro

Protein Sequence (636 amino acids)

>A4249_RS15715 phosphomethylpyrimidine synthase ThiC (Brevundimonas sp. GW460-12-10-14-LB2)
MNIQVTPPALPEEPNVDLALKDAREQVLKETGTIPTGERAGSRKVYVAGELYPDIRVPFR
EVAVHASANEPPVTIYDSSGPYTDPTVTIDIKRGLPRVKSSWQLDRGDIAPVANPREVKP
EDNGHASGRHLAPRFDTSNHRVFKGVEGRPVTQYEYAQAGIITPEMEYVAIRENLRREQN
SPCIRDGEDFGAAIPDFVTPEFVRQEIARGRAIIPQNINHPEVEPMIIGRNFLVKINANI
GNSAVLSSVDDEVDKLVWATRWGADNVMDLSTGRNIHNIRDWIIRNSSVPIGTVPIYQAL
EKVNGVAEDLTWEVFRDTLIEQAEQGVDYFTIHAGVRLPFVPMTAKRVTGIVSRGGSIMA
KWCLAHHKESFLYEHFEDICDIMRAYDVSFSLGDGLRPGSIADANDEAQFAELRTLGELT
KIAWAKGCQVMIEGPGHVPMHKIKQNMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSAI
GAAMIGWFGTAMLCYVTPKEHLGLPDRQDVKDGVITYKIAAHAADLAKGHPAARLHDDAL
SRARFEFRWEDQFNLGLDPETARKYHDATLPKEAHKTAHFCSMCGPKFCSMKISQDIRDA
AAAQNDAGASLSEAEAGMAEMSAKFRAGGGVVEVKV