Protein Info for A4249_RS15355 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: DNA topoisomerase (ATP-hydrolyzing) subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 808 TIGR01059: DNA gyrase, B subunit" amino acids 11 to 805 (795 residues), 954 bits, see alignment E=2e-291 PF02518: HATPase_c" amino acids 38 to 183 (146 residues), 60.1 bits, see alignment E=7e-20 PF00204: DNA_gyraseB" amino acids 236 to 405 (170 residues), 184.3 bits, see alignment E=3.4e-58 PF01751: Toprim" amino acids 434 to 546 (113 residues), 66.3 bits, see alignment E=5.7e-22 PF21249: GyrB_hook" amino acids 548 to 572 (25 residues), 35.6 bits, see alignment (E = 1.4e-12) PF00986: DNA_gyraseB_C" amino acids 736 to 798 (63 residues), 98.1 bits, see alignment 6.3e-32

Best Hits

Swiss-Prot: 77% identical to GYRB_CAUVC: DNA gyrase subunit B (gyrB) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 87% identity to bsb:Bresu_0006)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I234 at UniProt or InterPro

Protein Sequence (808 amino acids)

>A4249_RS15355 DNA topoisomerase (ATP-hydrolyzing) subunit B (Brevundimonas sp. GW460-12-10-14-LB2)
MTDPIADLPEYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVYEVVDNAIDEALAG
HADLVQVILNSDGSVTVTDNGRGIPTGIHEGEGVSAAEVIMTQLHAGGKFDQNSYKVSGG
LHGVGVSVVNALSDWLELKIYRDGKAHQMRFERGDTVKSLEVTGDAPIREDTGKPLSGTE
VTFFPSVTTFSHIDFDLKTLEHRLRELAFLNSGVVIKLADQRHAEPVEMILHYEGGVEAF
VRHLDKSKTPILKDVIVIRGKKEGIELDLALWWNDSYHETMLCFTNNIPQRDGGTHLSAF
RASLTRVMGGYIESSGAGKKEKVSVTGEDAREGLTCVLSVKVPDPKFSSQTKDKLVSSEV
RPAVESLCSEGLATWFEEHPVEAKQIVAKIIEAASAREAARKARDLTRRKSALEISSLPG
KLADCQERDPAKSELFIVEGDSAGGSAKQARNRENQAVLPLRGKILNVERARFDRMLSSD
LIGTLILALGTGIGRDDFNADKLRYHKIILMADADVDGAHIRTLLLTFFYRQMPELITRG
HVYIAQPPLYKVSKGKQSRYLKDQAEMDAYLIEEGASEAELDLSTGERRLGLDLQSLVRE
AKAFSAGVDRLSQRAPAFAIEQAALAGLFAEHSDMATASAAAAVRLNLYAEEGDGDWTGA
PGEQGAVVFTRTRRAVQETIVLEETLIRSLDARRLAERASAFDGVFVAPATYRRKDKSTT
IRGPLDLLTAVLDAGKKGLSIQRYKGLGEMNPEQLWETTLDVNARTLLQVSVEHEEDAND
LFAKLMGDVVEPRREFIQDNALDAAVDV