Protein Info for A4249_RS15000 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: endonuclease III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 247 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01083: endonuclease III" amino acids 36 to 225 (190 residues), 252.5 bits, see alignment E=1.2e-79 PF00730: HhH-GPD" amino acids 65 to 199 (135 residues), 78.4 bits, see alignment E=7.7e-26 PF00633: HHH" amino acids 131 to 157 (27 residues), 29.3 bits, see alignment (E = 8.1e-11) PF10576: EndIII_4Fe-2S" amino acids 218 to 234 (17 residues), 27.3 bits, see alignment (E = 5.2e-10)

Best Hits

Swiss-Prot: 57% identical to END3_RICCN: Endonuclease III (nth) from Rickettsia conorii (strain ATCC VR-613 / Malish 7)

KEGG orthology group: K10773, endonuclease III [EC: 4.2.99.18] (inferred from 81% identity to bsb:Bresu_0420)

Predicted SEED Role

"Endonuclease III (EC 4.2.99.18)" in subsystem Control of cell elongation - division cycle in Bacilli or DNA Repair Base Excision (EC 4.2.99.18)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I204 at UniProt or InterPro

Protein Sequence (247 amino acids)

>A4249_RS15000 endonuclease III (Brevundimonas sp. GW460-12-10-14-LB2)
MKPAKARKPAAKPAKRPAVMTGAAIPVLRWPPDEDRVEAIFKRLSGVMPEPKTELDFSNP
FTLVVAVALSAQATDVSVNKATERLFKVADTPEKMLALGEEGLIPYIASIGLYRGKARNV
IALSRIVLDQHGGEVPLNRADLQALPGVGRKTASVVLNELGIEAAIAVDTHVFRVSHRLG
LANAGTPDKVEAQLFKVVPEDWLPKAHHWLILHGRYTCTARKPKCLSCIIADLCPSRAGL
MALGETA