Protein Info for A4249_RS14720 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 PF00919: UPF0004" amino acids 16 to 119 (104 residues), 104.5 bits, see alignment E=3.8e-34 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 16 to 454 (439 residues), 465.3 bits, see alignment E=1.9e-143 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 17 to 457 (441 residues), 479.3 bits, see alignment E=1.2e-147 PF04055: Radical_SAM" amino acids 169 to 341 (173 residues), 97.1 bits, see alignment E=2e-31 PF01938: TRAM" amino acids 396 to 457 (62 residues), 25.3 bits, see alignment E=1.6e-09

Best Hits

Swiss-Prot: 71% identical to MIAB_PHEZH: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Phenylobacterium zucineum (strain HLK1)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 79% identity to bsb:Bresu_0233)

MetaCyc: 49% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A161I7M9 at UniProt or InterPro

Protein Sequence (478 amino acids)

>A4249_RS14720 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB (Brevundimonas sp. GW460-12-10-14-LB2)
MTETLVPQVETAPQKRLFIKTYGCQMNVYDSERMADVLRPLGYGVTDDVKAADFVILNTC
HIREKAAEKIYSELGKLREMRDIRRETGGDLTIAVAGCVAQAEGEEIMRRQPAVDIVVGP
QAYHQLPELLTRTARARGERIGADFAPDDKFDALPAARFTEGPTAFLTVQEGCDKFCTFC
VVPYTRGAEWSRPMAAVLEEARQLADRGVREVTLLGQNVNAYDGERPDGRKATLAELAYA
LAEIPGLDRIRYTTSHPNDMADELIAAHGDLDALMPYLHLPVQAGSDRILRAMNRKHGRQ
KYFDLIDRIRVARPDIAIAGDFIVGFPGETDREFEDTLDLVRRVNYAGAFAFAYSPRPGT
PAAGMGKQVEPNVVKDRLHRLIALLTEQQTAFNAAQAGRTLNVLFDKTGRHGHRRQAIGR
SPYLQSVHVDDADHLIGQIVPVEIIAGQQNSLSGRLIADGLKQPGRAGSATAQKEKAA