Protein Info for A4249_RS14615 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: hydroxymethylglutaryl-CoA lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00682: HMGL-like
  • amino acids 5 to 282 (278 residues), 135.7 bits, E=1.1e-43

Best Hits

KEGG orthology group: K01640, hydroxymethylglutaryl-CoA lyase [EC: 4.1.3.4] (inferred from 80% identity to bsb:Bresu_3234)

Predicted SEED Role

"Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism (EC 4.1.3.4)

MetaCyc Pathways

• L-leucine degradation I (5/6 steps found)
• mevalonate degradation (1/2 steps found)
• ketogenesis (3/5 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.4

Sequence Analysis Tools

See A0A161IJ36 at UniProt or InterPro

Protein Sequence (304 amino acids)

>A4249_RS14615 hydroxymethylglutaryl-CoA lyase (Brevundimonas sp. GW460-12-10-14-LB2)
MGRFIQIVEVGPRDGLQNEKAVLEPAVRADLVRRLEQAGAKRIEAVSFVHPKYVPQMAGA
EEVMAALPHEAGRSRIGLVLNGKGYDRALATGVDEVNVSVAATDGFGLKNQGLAVKDQLA
MLGEIVARRHNAEASEGAPSLSAMISCVWGCPFDGEVSVDQVADMVGAIAEMGVGEIGLA
DTIGAGDPWAVTKKVEAARAAAPDASLRLHFHDTRNTGLANAYAGVEAGIDVLDASVGGI
GGCPFAPGATGNIATEDLAYMLARGGFETGYDLDALIATARWIGEAIGRPAPSALSRAGG
FPKA