Protein Info for A4249_RS13960 in Brevundimonas sp. GW460-12-10-14-LB2
Annotation: alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to ARFB_PSEPU: Peptidyl-tRNA hydrolase ArfB (arfB) from Pseudomonas putida
KEGG orthology group: K15034, ribosome-associated protein (inferred from 84% identity to bsb:Bresu_0173)MetaCyc: 48% identical to peptidyl-tRNA hydrolase, ribosome rescue factor (Escherichia coli K-12 substr. MG1655)
Aminoacyl-tRNA hydrolase. [EC: 3.1.1.29]
Predicted SEED Role
"Hypothetical protein YaeJ with similarity to translation release factor"
Isozymes
Compare fitness of predicted isozymes for: 3.1.1.29
Use Curated BLAST to search for 3.1.1.29
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See A0A160HZM4 at UniProt or InterPro
Protein Sequence (134 amino acids)
>A4249_RS13960 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB (Brevundimonas sp. GW460-12-10-14-LB2) MTTVIPEDELEFHFYRAGGPGGQNVNKVSTAVQMRFDVKNTPSLTEPVKVRLMKLAGSRL TLEGVILITAVRHRTQERNRADAIERLQAMVDEASIRPVYRVPTRPTKASKERRLKAKTT RASIKSGRGKPSLD