Protein Info for A4249_RS13600 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: tryptophan--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 347 PF00579: tRNA-synt_1b" amino acids 14 to 292 (279 residues), 241.2 bits, see alignment E=8e-76 TIGR00233: tryptophan--tRNA ligase" amino acids 15 to 344 (330 residues), 316 bits, see alignment E=1.5e-98

Best Hits

Swiss-Prot: 74% identical to SYW_CAUVC: Tryptophan--tRNA ligase (trpS) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01867, tryptophanyl-tRNA synthetase [EC: 6.1.1.2] (inferred from 83% identity to bsb:Bresu_0290)

Predicted SEED Role

"Tryptophanyl-tRNA synthetase (EC 6.1.1.2)" (EC 6.1.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I1A8 at UniProt or InterPro

Protein Sequence (347 amino acids)

>A4249_RS13600 tryptophan--tRNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MTDQTPAVATFDGPRRILSGIQASGALHLGNYLGALKRFVELQDQGAPVYVFVADMHAIT
VWQDPAKLTAQTREIAAAYLASGLDPEKAIIFPQSAVRAHAELAWIFNCVARLGWLDRMT
QFKEKSGKHKERSSVGLYTYPVLQAADILLYKATEVPVGEDQKQHLELTRDIAAKFNNDF
GVAGFFPVPEPIIQGPATRVMSLRDGAAKMSKSDPSDNSRINLTDDADTIASKIKKAKTD
PEPLPETLDGLNERPEAKNLVAIYAALAGLTREQVIAQFGGQGFGAFKPALADLAVSSLA
PVTAEMRRLMDDPAEIDRVLKAGAERAAEVADPVVDEVKKIVGFWRA