Protein Info for A4249_RS13355 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 11 to 694 (684 residues), 1045.9 bits, see alignment E=0 PF01138: RNase_PH" amino acids 13 to 144 (132 residues), 116.7 bits, see alignment E=2.5e-37 amino acids 326 to 458 (133 residues), 100.5 bits, see alignment E=2.6e-32 PF03725: RNase_PH_C" amino acids 147 to 211 (65 residues), 53.9 bits, see alignment E=3.5e-18 amino acids 462 to 531 (70 residues), 25.2 bits, see alignment E=3.3e-09 PF03726: PNPase" amino acids 241 to 321 (81 residues), 54.8 bits, see alignment E=2.7e-18 PF00013: KH_1" amino acids 559 to 616 (58 residues), 45.1 bits, see alignment 1.7e-15 PF00575: S1" amino acids 621 to 693 (73 residues), 63.2 bits, see alignment E=6.2e-21

Best Hits

Swiss-Prot: 74% identical to PNP_PHEZH: Polyribonucleotide nucleotidyltransferase (pnp) from Phenylobacterium zucineum (strain HLK1)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 86% identity to bsb:Bresu_0214)

MetaCyc: 56% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160HZK5 at UniProt or InterPro

Protein Sequence (744 amino acids)

>A4249_RS13355 polyribonucleotide nucleotidyltransferase (Brevundimonas sp. GW460-12-10-14-LB2)
MFDIKRKTIDWAGRPLTLETGRIARQADGAVLATYGETVVLATVVYGRAPKPGLDFFPLT
VNYQEKTFAAGKIPGGYFKREGRPSEKETLVSRLIDRPIRPLFVKGFKNETQVVVTVLQH
DMENDPDVLGMVAASAALTISGVPFMGPIGAARVGLIDGELVLNPAIDLIPSSDLDLVVA
GTQDALMMVESEAKELSEEKMLEALMFAHAGMQPVIDAIIELAEHAAKEPFDFQADDHSD
VVASIKSLVGEDIKSAYTHAGKYERRSGVDAAKKAAAAKLVKTDENPDGVDGSKFSAAFK
ECEAEVLRRDIIENAHRVDGRALDKVRAIVSEVGVLPRTHGSALFTRGETQALVVATLGT
GEDEQYIDSLQGTYKENFLLHYNFPPYSVGEAGRMGSPGRREIGHGKLAWRAIRPMLPTK
EDFPYTIRLVSEITESNGSSSMATVCGSSLALMDAGVPLIRPVSGIAMGLILEPSGEFAI
LSDILGDEDHLGDMDFKVAGTSEGITSLQMDIKVAGITKEIMKQALTQASAGRLHILDEM
SKAMDAPRAELGEHAPKIETIKIPVDKIREVIGSGGKVIREIVEKTGAKIDIGDDGTIKV
AANDQEKIDAAKAWIQSIASEPEVGTIYSGKVVKVVDFGAFVNFFGAKDGLVHVSQIALE
RVANPADVLSEGQEVKVKFLGFDDRGKTKLSMKVVDQATGEDLTAKINAERAERGEEPLS
EDTGGRPKRDGGDRGGDRGRRRRD