Protein Info for A4249_RS13195 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: alpha-ketoacid dehydrogenase subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 374 PF02779: Transket_pyr" amino acids 39 to 213 (175 residues), 136.2 bits, see alignment E=9.9e-44 PF02780: Transketolase_C" amino acids 245 to 362 (118 residues), 123 bits, see alignment E=7.2e-40

Best Hits

Swiss-Prot: 65% identical to ODBB_PSEAE: 2-oxoisovalerate dehydrogenase subunit beta (bkdA2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00167, 2-oxoisovalerate dehydrogenase E1 component, beta subunit [EC: 1.2.4.4] (inferred from 88% identity to bsb:Bresu_3330)

Predicted SEED Role

"Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.2.4.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.4.4

Use Curated BLAST to search for 1.2.4.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A168NYT2 at UniProt or InterPro

Protein Sequence (374 amino acids)

>A4249_RS13195 alpha-ketoacid dehydrogenase subunit beta (Brevundimonas sp. GW460-12-10-14-LB2)
MSEQATFTDADRNDGVEPDMVDQNAAPASEATGAGVVPMNMIQALNSAIDVKMAEDANVL
SFGEDAGYFGGVFRVTDKLQQKHGLTRSFDAPISECGIVAAAIGMGAYGLRPVVEIQFAD
YIYPAYDQIVSEAAKMRYRSGGQFTSPITVRSPYGGGIFGGQTHSQSPESLFTHIAGLKV
VIPSNPYDAKGLLTAAIEDDDPVVFFEPKRLYNGPFDGWHEKPVSPWKAQDLAQVPTGKY
VEPIGKARVMREGNDVTILAYGTMVWVALAGAEHAGVDAEVIDLRSLVPLDIEAIEASVK
KTGRCVIVHEAPKTSGFGGELSALVQERCFYSLEAPIARVTGWDTPYPHAFEWEYFPGPQ
RVADALKSVMTGGR