Protein Info for A4249_RS13190 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: 2-oxo acid dehydrogenase subunit E2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 551 PF00364: Biotin_lipoyl" amino acids 6 to 76 (71 residues), 61.5 bits, see alignment E=2e-20 amino acids 129 to 199 (71 residues), 67.8 bits, see alignment E=2.2e-22 PF02817: E3_binding" amino acids 262 to 294 (33 residues), 51.6 bits, see alignment (E = 3e-17) PF00198: 2-oxoacid_dh" amino acids 321 to 549 (229 residues), 265.6 bits, see alignment E=1.3e-82

Best Hits

Predicted SEED Role

"Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168)" in subsystem Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 2.3.1.168)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.168

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (551 amino acids)

>A4249_RS13190 2-oxo acid dehydrogenase subunit E2 (Brevundimonas sp. GW460-12-10-14-LB2)
MGRFVFKLPDVGEGTAEAELVGWHVKVGDTVAEDQIVADVMTDKATVEITAPVSGKVVAL
HGEPGAMVPVRGPLVEFEVEGAGNADDAPTPVAPAAAKPDPAPVIAESPKVETLAAPAVE
APTSGNYVFKLPDVGEGTAEAELVGWHVKVGDAVEEDQIIADIMTDKATVEITSPVAGTV
VALYGEAGKSVPVGGPLVAFDVEGRGNVSTPVAAPKPASKPTENATTASAAISTAQSATS
ATALSKPVPALTGRAPGERPSASPAVRNRARDLGVDLTFVPGSGPAGRITHEDLDGFIAR
GGSQPAPAPSGGGSTYAKAQGTTEVKIIGLRRKIAEKMAESVRRIPHITYVEEIDMTAVE
ELRAHLNATKQKDQPKLNLLPFIARAIVVALRDQPQINATYDDEAGVLTQHAPVHLGIAA
QTPNGLMVPVVRHAEARDPYDTALEIARVSGAAKDGSAKREELSGSTITITSLGTLGGVV
HTPIINHPEVAILGPNKIAERVVVRDGQMVVRKMMNLSSSFDHRIVDGHDAAVFVQRIKG
LLEHPATLWMG