Protein Info for A4249_RS12770 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 18 to 895 (878 residues), 1355.7 bits, E=0
• PF00330: Aconitase
  • amino acids 78 to 562 (485 residues), 567.5 bits, E=2.7e-174
• PF00694: Aconitase_C
  • amino acids 692 to 819 (128 residues), 157.6 bits, E=2.2e-50

Best Hits

Swiss-Prot: 67% identical to ACNA_BRADU: Aconitate hydratase A (acnA) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 77% identity to aex:Astex_0445)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• TCA cycle III (animals) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• mixed acid fermentation (10/16 steps found)
• methylaspartate cycle (12/19 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See A0A160HZ58 at UniProt or InterPro

Protein Sequence (908 amino acids)

>A4249_RS12770 aconitate hydratase AcnA (Brevundimonas sp. GW460-12-10-14-LB2)
MPSIDSFKTRQDLSVGRKKYAYYSLPAAEEAGLTGISRLPRSMKVLLENLLRNEDGVSVT
EDDLKAVAAWVENKGSVEHEIAFRPARVLMQDFTGVPAVVDLAAMRDAMSALGADAAKIN
PLVPVDLVIDHSVMVDNFGTSKAFGQNVEREYERNIERYNFLRWGSSAFNNFRVVPPGTG
ICHQVNLENLAQTVWTLDEGKKTVAYPDTVVGTDSHTTMINGLAVLGWGVGGIEAEAAML
GQPIPMLIPEVIGFKLTGAMPEGTTATDLVLTVTQMLRKKGVVGKFVEFFGDALPNMTIE
DQATIANMAPEYGATCGFFPVSAATIGYLTATGRDKARVALVEAYAKAQGLWIDENSEDP
VFTDVLELDISTVVPSLAGPKRPQDRVELTTAAPAFETALTDVFARPSDAERVSVDGQKF
TVGDGDVVIAAITSCTNTSNPSVLIAAGLVARKANALGLKAKPWVKTSLAPGSQVVTDYL
TDAGLQKDLDALGFNLVGYGCTTCIGNSGPLDPAISKAINDNALVATSVLSGNRNFEGRV
NPDVQANYLASPPLVVAYAIAGSMRIDITKDPIGQDKKGNDVFLKDVWPTTQEIADIQRK
SVTPAMFAKRYKDVFKGDKHWQAIKVTGGQTYEWDDSSTYVANPPYFDGLSMDLTPVQDV
VEARVLAIFGDSITTDHISPAGSIKKTSPAGVYLTNHGVEAAEFNSYGARRGNHEVMMRG
TFANIRIKNRITPEIEGGVTKHFPSGDTMSIYDAAMRYQSEGRPLVVFAGKEYGTGSSRD
WAAKGTRLLGVRAVIAESFERIHRSNLVGMGVVPLQFKQDGWLKLGLTGEEIVTIRGLSD
ANIGKLKPRQDLWVELFRPSDGKMARFPVRCRIDNQTELDYLLAGGVMPYVLRNLARGPE
TEAPIAAE