Protein Info for A4249_RS12405 in Brevundimonas sp. GW460-12-10-14-LB2

Annotation: histidine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 TIGR00442: histidine--tRNA ligase" amino acids 13 to 463 (451 residues), 368.8 bits, see alignment E=1.8e-114 PF13393: tRNA-synt_His" amino acids 16 to 368 (353 residues), 149.3 bits, see alignment E=1.5e-47 PF03129: HGTP_anticodon" amino acids 387 to 461 (75 residues), 41.9 bits, see alignment E=8.9e-15

Best Hits

Swiss-Prot: 68% identical to SYH_CAUVC: Histidine--tRNA ligase (hisS) from Caulobacter vibrioides (strain ATCC 19089 / CB15)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 82% identity to bsb:Bresu_0269)

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A160I166 at UniProt or InterPro

Protein Sequence (496 amino acids)

>A4249_RS12405 histidine--tRNA ligase (Brevundimonas sp. GW460-12-10-14-LB2)
MTDAAPPRPLARNPRGFADKRGRDLTAERRIVARVSEVYERWGFEPLETPAFEYADALGK
FLPDADRPNEGVFALQDDAGSDEPGEWMALRYDHTAPLARFAAQTWETLPKPFRRYAYGP
VWRNEKPGPGRFREFWQCDADTVGSDRPEADAEIIAMGCEGLRAAGLDAGQAQIRVSNRK
LFDGLFDAGGVTEAGQRLTALRAIDKFDRLGWEGVVQLLGEGRLDDSGDYTKGAQLPAKV
TAAIEAFLASANTPGLSRAETLDAVARSGDLGAAGEAALNELAAIDRALAAMSVGQDAVK
FDPTIVRGLEYYTGAVFEAELLLDTVDDKSRPVRFGSIGGGGRYDDLVARFTGQSTPATG
FSFGVSRLASALRAAGRGAEDAVRGPVVVIVFSEDDMAHYLTAVAELRAAGIAAELYLGR
AGMKAQMKYADRRGAPAVVILGGDEIAAGEVTIKDLDAGRAAAAAITDNDAWKAERPGQM
KVPREGLVAAIRSIIE